House Secondary Banner
 



        Red Fluorescent Protein (RFP) Antibodies


Red Fluorescent Protein (RFP) Antibody Overview

 

 

Fluorescent proteins such as green fluorescent protein (GFP) or Discosoma Red Fluorescent Protein or DsRFP are widely used in research practice. Fusion RFP and GFP commonly serve as marker for gene expression and protein localization. While GFP is originated from Aequorea victoria jellyfish, DsRed was isolated from sea anemone Discosoma sp. mushroom. As GFP and DsRed share only 19% identity, therefore, in general, anti-GFP antibodies do not recognize DsRed protein and vice versa. Structurally, Discosoma red fluorescent protein is similar to Aequorea green fluorescent protein in terms of its overall fold (a β-can) and chromophore-formation chemistry. However, Discosoma red fluorescent protein undergoes an additional steps in the chromophore maturation and obligates tetrameric structure.
 
Using site-directed mutagenesis, a number of DsRed protein variants have been created allowing the red fluorescent protein mature as a monomeric form. Among DsRFP monomeric variants are monomeric mutant mRFP1, mBanana, mCherry, mHoneydew, mPlum, mOrange, mStrawberry and mTangerine that offer a wide range of fluorescent colors. As Rockland RFP polyclonal antibodies are raised against whole RFP protein of wild type, the polyclonal antibodies are expected to recognize all RFP variant forms. All Rockland Immunochemicals RFP antibodies are affinity-purified RFP assuring both high affinity and specificity. RFP polyclonal antibodies have been raised in rabbit and chicken hosts. Mouse monoclonal RFP antibodies and RFP recombinant control protein are also available. All RFP antibodies have been pre-absorbed to eliminate any potential cross-reactivity to human, mouse and rat serum proteins. The antibodies are also confirmed for non-reactivity to GFP protein. In addition, we performed conjugation of RFP antibodies to either fluorescent dyes, biotin or horseradish peroxidase to further facilitate RFP protein detection and quantification. 
 

 

 

 

 

 

 

 


 

Suggested Products for Common Assays

 

For ELISA (E) use Biotin Conjugated Anti-RFP (Chicken) Antibody in sandwich or titer ELISA formats. This affinity-purified antibody will yield excellent signal to noise ratio when used in combination with streptavidin peroxidase.

 

For Western Blotting (WB) use Peroxidase Conjugated Anti-RFP (Rabbit) Antibody for colorimetric substrate and chemiluminescent substrate detection. Use our blocking buffer to reduce background and to enhance sensitivity.

 

For Immunofluorescence Microscopy (IF) use Fluorescein Conjugated Anti-RFP (Rabbit) Antibody. This antibody will recognize all forms of RFP variants and is ideal as a general use anti-RFP antibody for fluorescence based immunoassays including FLISA.

 

For Immunohistochemistry (IHC) use Peroxidase Conjugated Anti-RFP (Rabbit) Antibody. HRP conjugates can be used in combination with MaxTag™ DAB tablets for immunohistochemistry.

 

 


 

 

The Rockland Advantage: Anti-RFP Antibodies

 

All Rockland Immunochemicals RFP antibodies are affinity-purified to assure both high affinity and specificity. Rigorous quality control testing ensures that the finished product meets or exceeds out high standards for optimum performance in your assays. RFP polyclonal antibodies raised in rabbit and chicken hosts against whole RFP recombinant proteins recognize all RFP variants.

 

All RFP antibodies have been pre-absorbed to eliminate any potential cross-reactivity to human, mouse and rat serum proteins. The antibodies are also confirmed for non-reactivity to GFP protein.

 

 


 

Red Fluorescent (RFP) Primary Antibodies

 

 

Name Catalog # Applications Size

 

RFP Antibody

 

600-901-379  ELISA, WB, IHC 100 μg

 

RFP Antibody Pre-adsorbed

 

600-401-379 ELISA, WB, IHC    100 μg

 

RFP Antibody

 

200-301-379 ELISA, WB, IHC 100 μg

 

RFP Antibody Peroxidase Conjugated

 

600-403-379 ELISA, WB, IHC 100 μg

 

RFP Antibody Biotin Conjugated Pre-adsorbed    

 

600-406-379 ELISA, WB, IHC 100 μg

 

RFP Antibody Fluorescein Conjugated 

 

600-402-379 FLISA, WB, IHC 100 μg

 

RFP Chemiluminescent Western Blotting Kit

 

KCA379 WB Kit

  

 

View All RFP Primary Antibodies

 

 

RFP Antibody Applications

 

For more information or to learn more about how Rockland’s Anti-GFP solutions can meet your needs, contact us today.

 

 

Reference Rockland Bucket

 

   

 

 

New Lab Set Up