Intercellular Matrix

The intercellular matrix (ICM), also known as extracellular matrix (ECM), represents a complex and dynamic network that envelops and provides structural support to cells in multicellular organisms. Composed of a diverse array of proteins, often referred to as the matrisome, the ICM plays a pivotal role in maintaining tissue integrity and regulating cellular behavior.

Figure. Structure of the Intercellular Matrix

Collagen, the most abundant ICM protein, forms fibrils that confer tensile strength to tissues. Elastin provides elasticity, enabling tissues to stretch and recoil. Fibronectin and laminin function as adhesive molecules, facilitating cell attachment and migration. Proteoglycans, such as hyaluronic acid, contribute to the gel-like properties of the ICM, while also aiding in cell signaling and hydration. Additionally, enzymes called matrix metalloproteinases (MMPs) regulate ICM remodeling by degrading and modifying its components. Through intricate interactions with cells, the ICM influences various biological processes including cell proliferation, migration, and differentiation. Understanding the complex interplay between cells and the ICM is crucial for unraveling fundamental mechanisms underlying tissue development, homeostasis, and disease progression.

Collagens: The Core ICM Component

Collagen, making up to 30% of the total protein mass in multicellular animals, is the predominant fibrous protein found in the ICM. Discover trusted collagen antibodies and proteins for precise detection and analysis, backed by citations in over 300 research publications.

Other Core ICM Components

Over 300 different proteins comprise the core matrisome (See Hynes & Naba 2012 and Naba et al. 2012 for comprehensive reviews). The following  antibodies enable the accurate detection, quantification, and visualization of core ICM components in tissue samples and cultured cells.

ICM Remodellers

The ICM undergoes continuous remodeling as its components are deposited, degraded, or modified. The following antibodies target proteins involved in ICM remodelling.

Purified Collagenase Proteins

Collagenase, purified from Clostridium histolyticum, serves as a potent enzymatic tool, facilitating the controlled degradation of collagen-based intercellular matrices.

References

1. Dzobo, K., Motaung, K. S. C. M., & Adesida, A. (2019). Recent Trends in Decellularized Extracellular Matrix Bioinks for 3D Printing: An Updated Review. International journal of molecular sciences, 20(18), 4628.
2. Hynes, R. O., & Naba, A. (2012). Overview of the matrisome--an inventory of extracellular matrix constituents and functions. Cold Spring Harbor perspectives in biology, 4(1), a004903. 
3. Naba, A., Clauser, K. R., Hoersch, S., Liu, H., Carr, S. A., & Hynes, R. O. (2012). The matrisome: in silico definition and in vivo characterization by proteomics of normal and tumor extracellular matrices. Molecular & cellular proteomics : MCP, 11(4), M111.014647.