House Buffer
 



        Protein A / Protein G Binding Reactivities


  

Polyclonal IgG Protein A Protein G Protein A/G
Bovine W S S
Bovine IgG1  nb S S
Bovine IgG2 S S S
Cat S W S
Chicken nb W nb
Dog S W S
Dog IgA (some) S W  --
Dog IgM (some) S W  --
Donkey M S S
Goat W S S
Goat IgG1 nb W S
Goat IgG2 S W S
Guinea Pig S W S
Guinea Pig IgG1 S W  --
Guinea Pig IgG2 S W  --
Hamster W W  --
Horse W S S
Horse IgG (ab) W nb W
Horse IgG (c ) W nb W
Horse IgG (T) nb S S
Human S S S
Human IgA W nb W
Human IgA1 W M M
Human IgA2 S nb  --
Human IgD nb nb W
Human IgE M nb M
Human IgG1 S S S
Human IgG2 S S S
Human IgG3 nb S S
Human IgG4 S S S
Human IgM (some) W nb W
Monkey (Rhesus) S S S
Mouse S S S
Mouse IgG1 W M M
Mouse IgG2a S S S
Mouse IgG2b S S S
Mouse IgG3 W S S
Mouse IgM nb nb nb
Rabbit S S S
Rat W M M
Rat IgG1 W M M
Rat IgG2a nb S S
Rat IgG2b nb W W
Rat IgG2c W W/S M
Sheep W S S
Sheep IgG1 nb S S
Sheep IgG2 S S S
Swine S W S
Swine IgA (some) S W  --
Swine IgM (some) S W  --

 

 

S = Strong binding

M = Medium binding

W = Weak binding

nb = no binding

w/s = weak overall, but affinity greater than protein A

-- = no data

 

 

 

 

Binding of immunoglobulins to Protein A and G is pH dependent.

Compiled from various sources.