Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody

ELISA results of Rabbit Anti-SARS-CoV-2 Nucleocapsid Protein Antibody.

Surface Plasmon Resonance (SPR) Data of Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody

ELISA results of Rabbit Anti-SARS-CoV Nucleocapsid (N) Protein Variants.

Fitting of Kinetic Data of Anti-SARS CoV Nucleocapsid

Binding mode and kinetic analysis of Anti-SARS-CoV Nucleocapsid

Datasheet

Sars Nucleocapsid Protein Antibody

Name: Sars Nucleocapsid Protein Antibody
Brand: Rockland Immunochemicals
Price: 475.0 USD
Availability: InStock

Rabbit Polyclonal

5 References

Item No. 600-401-A50

Size: 100 µg

Applications: ELISA, IF, Other

Reactivity: SARS-CoV

Host Species: Rabbit

$475.00 /Per Item

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Background

The coronavirus nucleocapsid protein is the major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid. Sequence comparison of the N genes of five strains of the coronavirus mouse hepatitis virus suggests a three-domain structure for the nucleocapsid protein. Anti-SARS-CoV Nucleocapsid (N) Protein Antibody is useful for researchers interested in viral research.

Expand Background

Specifications for Sars Nucleocapsid Protein Antibody

Product Details

Description: Anti-SARS-CoV Nucleocapsid (N) Protein (RABBIT) Antibody - 600-401-A50

Synonyms: rabbit anti-Sars Nucleocapsid Protein Antibody, rabbit anti-Sars-CoV Nucleocapsid (N) Protein Antibody, N antibody, N structural protein antibody, COVID19 antibody, Nucleocapsid protein antibody, Nucleoprotein antibody, SARS coronavirus N protein antibody, SARS CoV-2 antibody, SARSCoV2 antibody, Severe acute respiratory syndrome antibody, A50 Antibody, A50 SARS, COVID

Host Species: Rabbit

Clonality: Polyclonal

Format: IgG

Target Details

Reactivity: SARS-CoV

Immunogen Type: Recombinant Protein

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a purified recombinant protein corresponding to full length SARS Coronavirus Nucleocapsid protein.

Purity/Specificity: Anti-SARS-CoV-1 (N) affinity purified antibody is directed against SARS Coronavirus Nucleocapsid (N) protein. The product was purified from monospecific antiserum by immunoaffinity chromatography over SARS CoV-2 (N) resin. Detects the Nucleocapsid (N), Omicron, and BA.2 sub-variant. BLAST analysis was used to suggest reactivity with related Coronavirus proteins. Cross reactivity with homologues from other sources has not been determined.

Database Links:

UniProtKB - P59595

NCBI - 30173007

GeneID - 1489678

Application Details

Tested Applications: ELISA, IF

Suggested Applications: Other - View References

Application Note: Anti-SARS-CoV-1 purified antibody has been tested for use in ELISA, immunofluorescence, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 46 kDa in size corresponding to SARS Nucleocapsid (N) protein by western blotting in the appropriate cell lysate or extract. This antibody can be useful in ELISA and lateral flow format to detect virus in extracts from nasal and throat swabs and saliva. IF can be been useful to determine the presence or absence of virus entering cells especially when anti-viral drugs are applied. IHC studies can be performed on biopsies, detecting the coronavirus in lung, liver, bile duct, and placenta tissue. And may have the ability to neutralize the virus and thereby protect cells from the uptake of live virus. This antibody may be useful in flow cytometry studies.

Formulation

Physical State: Liquid (sterile filtered)

Concentration: 1.2 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

References (5)

Zhao B. et al. (2020). Recapitulation of SARS-CoV-2 Infection and Cholangiocyte Damage with Human Liver Organoids. Protein Cell

Applications

IF, Confocal Microscopy

PubMed

Burbelo P.D. et al. (2020). Detection of Nucleocapsid Antibody to SARS-CoV-2 is More Sensitive than Antibody to Spike Protein in COVID-19 Patients. medrxiv

Applications

Protein Assay

PubMed

Liu Y. et al. (2020). Functional and Genetic Analysis of Viral Receptor ACE2 Orthologs Reveals Broad Potential Host Range of SARS-CoV-2. Proc Natl Acad Sci U S A

Applications

IF, Confocal Microscopy

PubMed

V’kovski P. et al. (2020). Disparate temperature-dependent virus – host dynamics for SARS-CoV-2 and SARS-CoV in the human respiratory epithelium. PLoS Biol.

Applications

IF, Confocal Microscopy

PubMed

Grant BD, Anderson CE, Williford JR, et al. (2020). A SARS-CoV-2 Coronavirus Nucleocapsid Antigen-Detecting Half-Strip Lateral Flow Assay Towards the Development of Point of Care Tests Using Commercially Available Reagents. Anal Chem.

Applications

Other

PubMed

Related Protocols

ELISA Protocol

Immunocytochemistry (ICC) Protocol

Immunofluorescence (IF) Protocol

Sandwich ELISA Protocol for Collagen

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.