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        Notch Signaling


Notch Signaling Pathway Overview

The Notch pathway is a fundamental signaling system allowing neighboring cells to communicate and perform their proper developmental role. Notch proteins mediate critically important cellular functions through direct cell-cell contact. Notch signaling involves gene regulation mechanisms that control multiple cell differentiation processes during embryonic and adult life. Rockland’s Notch antibodies help scientists to understand cell communication, growth and function regulation.








View All Notch Signaling Pathways


Notch signaling also has a role in:

  • neuronal function and development
  • stabilization of arterial endothelial fate and angiogenesis
  • regulation of crucial cell communication during formation of the valve primordial and ventricular development and differentiation
  • cardiac valve homeostasis
  • timely cell lineage specification of both endocrine and exocrine pancreas
  • influencing of binary fate decisions of cells
  • expansion of the hematopoietic stem cell compartment during bone development
  • T cell lineage commitment from common lymphoid precursor

Faulty notch signaling is implicated in many diseases including T-ALL (T-cell acute lymphoblastic leukemia), CADASIL (Cerebral Autosomal Dominant Arteriopathy with Sub-cortical Infarcts and Leukoencephalopathy), MS (Multiple Sclerosis), Tetralogy of Fallot, Alagille syndrome, and many cancers.




Research

Notch signaling pathways are a booming area of pharmacological research, due largely to the direct connection to human disease intervention. Notch receptor activating mutations leading to nuclear accumulation of NICD are common in adult T-cell acute lymphoblastic leukemia and lymphoma. In addition, loss-of-function Notch receptor and ligand mutations are implicated in several disorders, including Alagille syndrome and CADASIL, an autosomal dominant form of cerebral arteriopathy. Research utilizing antibodies and proteins found within the notch signaling pathway holds great promise in cancer treatment as well, making these products highly valued in the scientific community.



The Rockland Notch Antibody Advantage

Rockland products can help in a variety of Notch signaling research areas. Notch receptors are single-pass trans-membrane proteins composed of functional extracellular (NECD), transmembrane (TM), and intracellular domains. In the signal-receiving cell, γ-secretase (also involved in Alzheimer’s disease) releases the NICD from the TM (S3 cleaved notch), which translocates to the nucleus where it associates with the CSL (CBF1/Su(H)/Lag-1) family transcription factor complex, resulting in subsequent activation of the canonical csl notch target genes Myc, p21 and HES-family members.



Notch Antibodies

 

PRODUCT NAME

CATALOG#

APPLICATIONS 

SIZE 


 
Anti-NOTCH 1 (intra) (RABBIT) Antibody 100-401-405 ELISA, WB, IHC 200 µL

 
Anti-NOTCH 2 (intra) (Human specific) (RABBIT) Antibody 100-401-406 ELISA, WB, IHC 200 µL

 
Anti-NOTCH 1 (Cleaved N terminal) (Human specific) (RABBIT) Antibody 100-401-407 ELISA, WB 200 µL
   Trial Size 100-401-407S ELISA, WB 25 µL

 
Anti-NOTCH 2 (Cleaved N terminal) (Human specific) (RABBIT) Antibody 100-401-408 ELISA, WB, IHC 200 µL
   Trial Size 100-401-408S ELISA, WB, IHC 25 µL

 

 



Applications

Common applications for antibodies in the Notch signaling pathway include:  
 



TrueBlot® Mouse Western Blot Kit

 

TrueBlot Notch IP Western Blotting Kit  

 

The Mouse TrueBlot® Western Blot Kit contains the critical supporting reagents, buffers, and substrates for Notch immunoprecipitation and Western blotting of samples using TrueBlot second step immunoblotting reagents in conjunction with your own primary IP antibody and primary (Mouse IgG) Western blotting antibody. TrueBlot technology enables unhindered detection of protein bands of interest which would otherwise be obscured by the presence of reduced and denatured heavy and light chain immunoglobulin in the blot (as detected by the conventional immunoblotting HRP anti-mouse IgG reagent). Mouse IgG TrueBlot® ULTRA is the unique horseradish peroxidase conjugated anti-mouse IgG immunoblotting second step reagent which enables detection of Notch immunoblotted target protein bands, without hindrance by interfering immunoglobulin heavy and light chains from your IP antibody. Use it in place of your usual HRP anti-mouse IgG immunoblotting second step reagent. It is easy to generate publication-quality Notch IP Western Blot data with Mouse IgG TrueBlot® ULTRA.

 

 

Mouse IgG TrueBlot ULTRA is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of mouse IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Mouse IgG TrueBlot ULTRA with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in Notch IP Western Blotting applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions.

  

Notch IP / Western Blotting comparison of TrueBlot and conventional reagents