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        NOTCH Signaling


Notch Signaling Pathway Overview

 

The NOTCH pathway is a fundamental signaling system allowing neighboring cells to communicate and perform their proper developmental role. NOTCH proteins mediate critically important cellular functions through direct cell-cell contact. NOTCH signaling involves gene regulation mechanisms that control multiple cell differentiation processes during embryonic and adult life. Rockland’s NOTCH antibodies help scientists to understand cell communication, growth and function regulation.








View All Notch Signaling Pathways


NOTCH signaling also has a role in:

  • neuronal function and development
  • stabilization of arterial endothelial fate and angiogenesis
  • regulation of crucial cell communication during formation of the valve primordial and ventricular development and differentiation
  • cardiac valve homeostasis
  • timely cell lineage specification of both endocrine and exocrine pancreas
  • influencing of binary fate decisions of cells
  • expansion of the hematopoietic stem cell compartment during bone development
  • T cell lineage commitment from common lymphoid precursor

Faulty NOTCH signaling is implicated in many diseases including T-ALL (T-cell acute lymphoblastic leukemia), CADASIL (Cerebral Autosomal Dominant Arteriopathy with Sub-cortical Infarcts and Leukoencephalopathy), MS (Multiple Sclerosis), Tetralogy of Fallot, Alagille syndrome, and many cancers.




Research

Notch signaling pathways are a booming area of pharmacological research, due largely to the direct connection to human disease intervention. Notch receptor activating mutations leading to nuclear accumulation of NICD are common in adult T-cell acute lymphoblastic leukemia and lymphoma. In addition, loss-of-function Notch receptor and ligand mutations are implicated in several disorders, including Alagille syndrome and CADASIL, an autosomal dominant form of cerebral arteriopathy. Research utilizing antibodies and proteins found within the notch signaling pathway holds great promise in cancer treatment as well, making these products highly valued in the scientific community.



The Rockland Notch Antibody Advantage

Rockland products can help in a variety of Notch signaling research areas. Notch receptors are single-pass trans-membrane proteins composed of functional extracellular (NECD), transmembrane (TM), and intracellular domains. In the signal-receiving cell, γ-secretase (also involved in Alzheimer’s disease) releases the NICD from the TM (S3 cleaved notch), which translocates to the nucleus where it associates with the CSL (CBF1/Su(H)/Lag-1) family transcription factor complex, resulting in subsequent activation of the canonical csl notch target genes Myc, p21 and HES-family members.



NOTCH Antibodies

 

PRODUCT NAME

CATALOG#

 

APPLICATIONS 

SIZE 


 
Anti-NOTCH 1 (intra) (RABBIT) Antibody

100-401-405

ELISA, WB, IHC

200 µL

 
Anti-NOTCH 2 (intra) (Human specific) (RABBIT) Antibody

100-401-406

ELISA, WB, IHC

200 µL

 
Anti-NOTCH 1 (Cleaved N terminal) (Human specific) (RABBIT) Antibody

100-401-407

ELISA, WB

200 µL
   Trial Size

100-401-407S

ELISA, WB

25 µL

 
Anti-NOTCH 2 (Cleaved N terminal) (Human specific) (RABBIT) Antibody

100-401-408

ELISA, WB, IHC

200 µL
   Trial Size

100-401-408S

ELISA, WB, IHC

25 µL

 

 

Applications

Common applications for antibodies in the Notch signaling pathway include:
 

Clickable Notch Pathway  

 

Clickable Notch Pathway

 

 

 

 

 

TrueBlot® Mouse Western Blot Kit

 

TrueBlot Notch IP Western Blotting Kit

   

The Mouse TrueBlot® Western Blot Kit contains the critical supporting reagents, buffers, and substrates for Notch immunoprecipitation and Western blotting of samples using TrueBlot second step immunoblotting reagents in conjunction with your own primary IP antibody and primary (Mouse IgG) Western blotting antibody.