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Loading controls are essential for the interpretation of assays. Ideal controls are expressed constitutively and at high levels with low variability between cell lines and experimental conditions. In western blot assays the loading control should be at a different molecular weight than the protein of interest this allows the protein to be visually distinguishable. Loading control antibodies not only allow the verification of equal protein loading between samples in western blot assays, but they also allow for identification of certain cell compartmentalization or cellular localization in Immunofluorescence Microscopy (IF) and Immunohistochemistry (IHC). Loading Control Antibodies recognize mostly housekeeping proteins in cells used in a scientific experiment. Rockland’s loading control antibodies are suitable for in assays including ELISA, FLISA, Western Blot, IF and IHC.
Anti-beta Actin (RABBIT)
Anti-alpha-Tubulin (MOUSE) Monoclonal
Anti-Nuclear Pore Complex (MOUSE)
For Western Blot (WB) use the Loading Control Antibody Sampler Kit -KAS-001. The Loading Control Antibody Sampler Kit contains antibodies to a variety of housekeeping proteins. This kit contains enough primary and secondary antibodies to perform ten western blots per loading control antibody. For IF assays use Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody 200-301-880.For Immunohistochemistry (IHC) use Anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (RABBIT) Antibody - 600-401-A33.For ELISA (E) use Anti-beta Actin (RABBIT) Antibody Biotin Conjugated - 600-406-886.
For Host Cell Protein detection (HCP) use Anti-Host Cell Protein Combined Biotin Conjugated (HCP) Antibody - 200-406-M61.
The Rockland Loading Control Antibodies Advantage Rockland incorporates scientific expertise with customized protocols and proven development methods to provide seamless and reliable loading control antibodies for any application. Rigorous quality control testing ensures that finished purified products meets or exceeds our high standards for optimum performance in your assays.
Rockland Immunochemicals Inc.Gilbertsville, PA 19525E-mail: email@example.comPhone: 800.656.7625