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While most fluorescent dyes adsorb and emit light in the visible or ultraviolet regions of the light spectrum, near infrared (NIR) dyes also called IRDYES offer clear advantages for fluorescence detection and imaging. Since light scattering and auto fluorescence are prominent in living systems, especially for in vivo imaging, it is important to work in spectral regions that are far from potential interference from endogenous fluorescence factors. This can be achieved working at NIR spectral regions (650-900 nm). Infrared dyes have absorption and emission wavelengths in the infrared and near-infrared (NIR) spectrum, between 680 and 800 nm. Additionally, fluorescent labels having NIR emission wavelengths have the ability to penetrate tissue deeper than other emission wavelengths, providing enormous potential for non-invasive imaging applications. For most biological applications, the stability of fluorescent dyes in combination with the appropriate buffers for the application is crucial for sustaining their functions over the course of a study.
Rockland produces IRDYE antibody conjugates for NIR imaging using DyLight®, ATTO-Tec and Cy dye. Rockland’s IRDYE secondary antibodies are often cross-adsorbed for multiplex detection and provide high specificity and are optimized for high sensitivity and excellent signal-to-noise performance in a variety of applications, including: immunoblotting, multiplex imaging, ELISA, microscopy and small animal in vivo imaging.
Featured IRDYE Antibody Conjugates
Anti-MOUSE IgG (H&L)(DONKEY) AntibodyDyLight™ 680 Conjugated610-744-002
Anti-RABBIT IgG (H&L)(GOAT) AntibodyDyLight™ 800 Conjugated(Min X Bv Ch Gt GP Ham Hs
Hu Ms Rt & Sh Serum Proteins)611-145-122
Fluorescent TrueBlot®: Anti-Mouse Ig DyLight™ 80018-4517-32
All Rockland IRDYE antibodies are designed be used by a variety of imaging systems that offer near-infrared excitation and emission such as LI-COR Odyssey™, UVP and Pearl™ Imaging Systems and other detection platforms. IRDYE antibodies combined with NIR fluorescent imaging delivers enhanced sensitivity due to low background autofluorescence in the near-infrared region yielding higher signal-to-noise ratios when used in immunoassays.
IRDYE dyes and their absorption and emission properties:
DyLight NIR dyes provide intense fluorescence and sensitivity requiring less conjugated antibody to study your cells.They are primarily suitable for use in immunofluorescence techniques such as microscopy, ELISA, Western blotting, high throughput screening and other array platforms.
Their absorption and emission wavelengths are compatible with popular fluorescence instrumentation. DyLight dyes are exceptional alternatives to Alexa Fluor®, CyDye® and IRDye® fluorescent dyes.
Due to their photostability and photophysical properties ATTO-TEC fluorescent labels are ideal for many bio-analytical applications. Absorption and fluorescence of the ATTO NIR dyes are optimized for the red region of the spectrum, with the advantage of efficiently suppressing or by-passing any auto-fluorescence of biological samples. ATTO-TEC labels also find many applications in fluorescence spectroscopy. All dyes are well characterized and can be used in time resolved spectroscopy, FRET (Fluorescence Resonance Energy Transfer) applications as well as single-molecule detection (SMD).
Cyanine dyes are a large family of dyes that, in general, contain an unsaturated carbon chain linked by heterocyclic rings such as, but not limited to indole, quinoline, isoquinoline, benzothiazole, and benzooxazole.
Rhodamines and oxazines have honeycomb-like structures since they are architectural clusters of hexagonal frameworks formed by conjugated π-systems. Because of their structure, this super family of dyes exhibits notable photostability and photophysical properties when compared to other families of dyes. Some well-known versions have common names like rhodamine 6G, rhodamine B, Alexa dyes, TAMRA, and Texas Red.
Fluorescent TrueBlot®, a new format of our popular TrueBlot® product line, combines the power and specificity of the original products with the versatility of fluorescent and near infra-red dyes. Conjugating highly optimized TrueBlot® reagents to a full spectrum of fluorescent labels, results in multipurpose reporter molecules that can be used in a variety of immunoassays.
Related Links • Fluorescent Western Blot Buffers• Western Blotting • Immunofluorescence Microscopy Protocol
Rockland Immunochemicals Inc.Limerick, PA 19468E-mail: email@example.comPhone: 800.656.7625