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Flow Cytometry for Secondary Antibodies


Flow Cytometry - Secondary Antibodies Overview


The use of secondary antibodies for flow cytometry has several advantages including amplification of the fluorescent signal and the possibility of using an unpurified and unlabeled primary antibody. Moreover, it also provides the opportunity to use biotinylated antibodies that can be detected using streptavidin conjugated to any of several fluorophores. Indirect staining with secondary antibodies can be easily combined with direct staining protocols using primary fluorophore-labeled antibodies and thereby seamless incorporated into multicolor staining experiments maintaining both specificity and flexibility.

Acclaimed secondary reagents for flow cytometry include Rockland’s FITC-, PE- and APC-conjugated IgG and F(ab')2 anti-mouse, anti-goat, anti-rabbit, anti-human and anti-rat antibodies. Also, for detection of biotinylated primary antibodies, FITC- and PE-conjugated streptavidin is the reagent of choice.



View all Secondary Antibodies for Flow


Featured Secondary Antibodies for Flow Cytometry



F(ab')2 Goat IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed - 705-708-125  





Mouse IgG (H&L) Secondary Antibody Fluorescein Conjugated Pre-Adsorbed - 610-102-121

F(ab')2 Anti-MOUSE IgG [H&L] (DONKEY) Antibody Phycoerythrin conjugated

Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins - 710-708-124

F(ab')2 Anti-MOUSE IgG (H&L) (GOAT) Antibody Phycoerythrin conjugated Min X Bv Hm, Hs, Hu Rb, Rt, & Sh Serum Proteins - 710-1832
Fluorescein Conjugated Affinity Purified Anti-MOUSE IgG (H&L) (GOAT) - 610-1202-0500




Anti-RABBIT IgG (H&L) (GOAT) Antibody Fluorescein Conjugated - 611-1202 
Anti-RABBIT IgG (H&L) (DONKEY) Antibody Fluorescein Conjugated - 611-7202






F(ab')2 Anti-HUMAN IgG F(c) (GOAT) Antibody Phycoerythrin conjugated

Min X Bv Hs Ms & Rt Serum Proteins - 709-1817 



Streptavidin Phycoerythrin Conjugated - S000-08



Reference Rockland Bucket




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