Leica has collaborated with Rockland Immunochemicals
to jointly analyze the sub-cellular localization of Akt using high-resolution STED microscopy.
Leica STED super-resolution microscopy allows for the investigation of cellular structural details beyond the resolution of conventional confocal microscopy. This has allowed an unparalleled ability to visualize cellular events. Indeed, STED imaging allowed for the first time to clearly associate the activated Akt with tubulin network early after EGF stimulation. Tubulin—a protein used by cells to form microtubules—interacting with Akt could facilitate its migration upon activation. As it is known that AKT plays an essential role in cancer cell signaling, this unprecedented direct observation could initiate and open an exciting new avenue in cancer research.