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        Custom Polyclonal Antibody Production


Rockland Immunochemicals has offered polyclonal antibody manufacturing services for more than four decades. Our expertise are offered using a variety of different hosts for the development of polyclonal antibodies, including the most commonly used New Zealand white rabbit, goats, sheep, guinea pigs, chickens, rats, mice and donkeys. As with our monoclonal antibody development service, Rockland’s custom antibody services allow you to direct the generation of your research required antibody. Should you – the researcher – require the development of a polyclonal antibody against a specific region or modified target epitope, let Rockland’s expertise aid you in the design and synthesis of your antigenic peptide, which upon conjugation to KLH will represent the starting antigen of your antibody development program. Should you choose to utilize a recombinant protein as primary antigen, our expertise in bacterial and insect cell-based recombinant protein expression will ensure production of antigen for immunization and screening regimen.


Customers are typically supplied with a pre-bleed serum (5 mL) for screening purposes prior to commencing with custom-based projects. Rockland’s experience and monitoring of the antibody-mediated immune response translates into recommendations discussed with the client prior to the collection of production bleeds. Upon approval, Rockland offers variable immunization schedules and use of different adjuvants on a custom basis and dependent on your need, can extend the collection of production bleeds on a monthly basis post the standard applied schedules show below.

 

 


Rapid Screen Protocol (1 month, 100mL yield from best responder)


 

Day - 4

Pre-bleed


 

Day 0

Immunize via an intradermal (ID) route using CFA as adjuvant


 

Day 7

Booster injection via an intradermal (ID) route using IFA as adjuvant


 

Day 14

Booster injection via an subcutaneous (SC) route using IFA as adjuvant


 

Day 28

Booster injection via an subcutaneous (SC) route using IFA as adjuvant


 

Day 38

Terminal Bleed (Client approved)


 

Day 38

Ship terminal bleed to client



Fast Protocol (100mL yield from best responder)


 

Day - 4 

Pre-bleed


 

Day  0

Immunize via an intradermal (ID) route using CFA as adjuvant


 

Day  7  

Booster injection via an intradermal (ID) route using IFA as adjuvant


 

Day 14

Booster injection via a subcutaneous (SC) route using IFA as adjuvant


 

Day 28 

Booster injection via a subcutaneous (SC) route using IFA as adjuvant 


 

Day 38 

Test Bleed           


 

Day 40

Ship pre-immune bleed and test bleed to client


 

Day 45 

Terminal Bleed (Client approved)


 

Day 47

Ship terminal bleed to client


 

Day 49

End of project (Researcher approved)   


 

PLEASE NOTE THAT ACTIVITY AFTER THE DAY 38 TEST BLEED WILL NOT BE PERFORMED WITHOUT APPROVAL BY THE CLIENT (e-mail or fax).



Standard Protocol (75mL yield per month per best responder)


 

Day - 4 

Pre-bleed


 

Day  0  

immunize via an intradermal (ID) route using CFA as adjuvant


 

Day  7  

Booster injection via an intradermal (ID) route using IFA as adjuvant   


 

Day 14 

Booster injection via a subcutaneous (SC) route using IFA as adjuvant                   


 

Day 28 

Booster injection via a subcutaneous (SC) route using IFA as adjuvant


 

Day 38 

Test Bleed           


 

Day 40 

Ship pre-immune bleeds and test bleeds to researcher


 

Day 45 

Production Bleed (Client approved)


 

Day 52 

Production Bleed (Client approved)       


 

Day 56 

Booster injection via a subcutaneous (SC) route using IFA as adjuvant                   


 

Day 66 

Production Bleed (Client approved)


 

Day 73 

Production Bleed (Client approved)                         


 

Day 75 

Booster injection via a subcutaneous (SC) route using IFA as adjuvant


 

Day 85 

Production Bleed (optional – if requested by client - additional charges apply)


 

Day 92 

Production Bleed (optional – if requested by client - additional charges apply)


 

Day 94 

Ship production bleeds to client


 

Day 98 

End of project (Client approved)


 
PLEASE NOTE THAT ACTIVITY AFTER THE DAY 38 TEST BLEED WILL NOT BE PERFORMED WITHOUT APPROVAL BY THE CLIENT (e-mail or fax).


Dependent on the selected protocol, terminal or production bleeds are collected according to the timelines shown above. Upon conclusion of the applied protocol, you – the researcher – are presented with the option of terminating the project for the collection of a terminal bleed (this only applies to rabbits) or by extending your study for the collection of additional bleeds on a monthly basis.


Collected sera can be pooled and purified using either Protein A or G protein and result in client-specified product vialing and shipment. Alternatively, Rockland’s expertise in antibody conjugation offers the labeling of your antibody with a chromogenic or fluorescent reporter of your choice.

 

Rockland has extensive experience in generating a wide range of custom polyclonal antibodies and our staff is always ready to assist you with experimental design, laboratory methods, production, conjugation and purification. If you have any questions about Custom Polyclonal Antibodies, please email our Custom Services Group  or call them at 1-800-656-ROCK.

 


Custom Services Frequently Asked Questions (FAQs).


We offer polyclonal antibody production in a wide variety of hosts, including Rabbit, Goat, Sheep, Guinea Pig, Chicken, Rat, Mouse, and Donkey.


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Additional Custom Polyclonal Antibody Services 


Code

 

Rapid Screen for Peptides  

 

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CUST61

 

This service is designed for the rapid screening of peptides usually delivered in about one (1) month! This is the fastest way to get custom polyclonal antibody for your immunoassay. This service includes analysis of the peptide's primary structure, peptide synthesis (up to 15 amino acids), conjugated to KLH carrier protein, pre-bleeding of two (2) rabbits, immunization, booster injections, terminal bleeding of rabbits and titer analysis of the antiserum collected by indirect ELISA. We will provide you with approximately 100ml of antiserum, the pre-immune serum and any remaining unconjugated peptide. Call for more details.

Pre-screening of host animal 

 

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CUST37

 

 

Host animals may show elevated titers in pre-immune sera to certain antigens of bacterial or fungal origin. If you are studying bacterial or fungal proteins you may request pre-screening of rabbits at no additional charge. Pre-bleed and daily maintenance fees apply to all host animals reserved for your selection. After the host animals are selected, the charge for animals would apply only to those host animals that meet your requirements.


Inquire for pricing and availability of other host animals including, but not limited to bovine, rat, guinea pig, hamster, turkey, donkey or swine. All of our customers are ensured ownership and confidentiality of each project.


Additional Laboratory Services for further processing of antiserum are also available.