Molecular Biology Services


Molecular Biology Services

 

Molecular biology products and services represent an invaluable tool for the discovery and development of tools and products in the life sciences and medical field. Rockland’s growing suite of molecular biology custom products and services will help accelerate your studies whether working on basic research or performing advanced translational studies. By directly interacting with our molecular biology scientists at every step of the process, you gain flexibility and reliability during the experimental design and execution of your project. Whether your needs are a simple nucleic acid purification or sequencing project, or assistance with design of a Chimeric Antigen Receptor (CAR) for a  CAR-T project, Rockland’s custom molecular biology services team is ready to help. Our Molecular Biology Services include:

 

 DNA Synthesis button    Gene Cloning Button    Protein Expression button
         
Protein purification button    DNA libraries button    Isolation of Nucleic Acids button
         
 Bioinformatics button    CRISPR button    Custom Services button



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 DNA Synthesis, Sequencing, Gene Design and Cloning

 

Oligonucleotides, gene fragments, even entire genes and plasmids, can be synthesized according to your custom sequence. Nucleic acids can also be fully sequenced per your specific requirements. Synthesized DNA fragments or provided DNA fragments can be cloned into plasmids or other forms of DNA.  

 

  • Oligonucleotide Synthesis  
  • Oligonucleotide Sequencing 
 
  • Vector Construction  
  • Plasmid Isolation using mini-prep system  
  • Plasmid Restriction Mapping  
 
  Gene Cloning

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Protein Expression and Purification  

 

Native and recombinant proteins can be purified from various sources. Recombinant gene constructions can be generated and produced in the expression system of your choice.

 

 

 E. coli
  • Bacterial protein expression 1L   
 
 Insect Cells
  • Recombinant Baculovirus  
  • Plaque assay for viral stock  
  • Baculovirus amplification using SF9  
  • Baculovirus MOI Optimization up to 6 small cultures 
  • Baculovirus protein expression up to 1L  
 
Mammalian Cells
  • Mammalian transient transfection  
  • Mammalian stable transfection up to five clones  
  • Mammalian retroviral transfection (transduction)  
  • Additional Colony expansions per clone  
  • Cell expansion and cryopreservation (up to 4 vials)  
  • Extended culture time for sample analysis 
  • Cell lysate service  
  • Cryopreservation of parental cell line (per clone up to 4 vials)  
  • Liquid nitrogen storage per clone per year up to 6 vials  
  • Master cell banking 
  • Mammalian protein expression  
  • Large Scale Production of Expressed Protein in HeLa cells 
  • 7 Liter batch bioreactor 
  • Media for bioreactor batch production 
  • Time Course and MOI (multiplicity of infection ) Assay 
 

 

 

 Protein Expression

 

Let us do your protein expression or send us the source material and the target protein will be thoroughly purified from contaminating proteins using a multi-step process that includes affinity chromatography.

 

  • Poly His Tag protein purification 1L  
  • GST protein purification 1L  
  • Custom protein purification 
  • Affinity purification 
  • HPLC purification  
  • HPLC Analysis  
  • Concentration by ultrafiltration  
  • Custom Lyophilization  
 
  Protein Purification


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DNA Libraries 


A cDNA library is generated in one of several available vectors using as little as 20 ng of mRNA.

  •  cDNA Library Construction  

 

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Purification and Isolation of Nucleic Acids 


Genomic DNA or mRNA is isolated from either frozen tissue or tissue culture cell preparations.

   

  • Genomic DNA isolation   
  • mRNA Isolation
  • cDNA Synthesis
 
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Bioinformatics 

 

  • Computational analysis  
  • Antigen consultation design of peptide or recombinant protein 
 
   Bioinformatics

 

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Other Molecular Biology Services 

 

 

Do you have a special molecular biology project but can’t find it on our Molecular Biology Services page? Please contact Customer Support for additional details and let us know how we can help.

 

 

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