How much antigen is required?

Using proprietary immunization methodologies, as little as 200 micrograms of antigen per injection can be used for a standard immunization protocol. However, we have been successful using approximately 50 micrograms per injection for valuable antigens.  If antigen is abundant, immunization with 0.1 to 0.5 mg per injection is highly recommended.

What is Rockland's recommended immunization regime?

Customized protocols can always be developed; however, we have documented a greater than 95% success rate by using the protocol listed above.

How many animals should I put on a study?

We recommend two or more animals to allow for immune response variability.

What type(s) of antigens can be injected?

We can immunize animals with native proteins, GST-fusion proteins, peptides, carbohydrates or any other compound of viral, fungal or microbial origin. However, no bio-hazardous material can be accepted. We recommend the use of Freund's adjuvant; however, can accommodate any requirements that you may have for specialized adjuvants.

Can you conjugate my peptide to a carrier?

Yes. Please consult with our technical staff to recommend peptide synthesis strategies to facilitate carrier coupling and enhance peptide immunogenecity.

How should I send the antigen?

Antigen can be supplied in a soluble form, as a lyophilizate, a gel slice or on a nitrocellulose membrane. An additional charge of $35 will be applied for gel slice extraction. Please call prior to shipping so that we can prepare for your project.

Is Rockland a USDA approved and NIH Licensed facility?

Yes. Our staff can assist you in completing necessary forms required by the NIH. Our in-house IACUC completes and maintains animal protocols eliminating your paperwork load.
USDA #23-R-134
NIH BPA #00008695
NIH Assurance OPRR #A4062-01