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To reduce variables in any type of experiment it is advisable to include both positive and negative controls in the experimental design. Negative controls are particular samples included in the experiment that are treated the same as all the other samples, but are not expected to change due to any variable in the experiment. The positive control sample will show an expected result, helping the scientist to understand that the experiment was performed properly. Some controls are specific to the type of experiment being performed such as molecular weight standards used in protein or DNA gel electrophoresis, i.e. SDS-PAGE or agarose gel electrophoresis. The proper selection and use of controls ensures that experimental results are valid and saves valuable time.
Rockland produces several different types of controls:
Loading Control Antibodies
Control Cell Lysates
Low Endotoxin Controls
Human IgA Fluorescein009-0206
Anti-Glyceraldehyde-3-Phosphate Dehydrogenase(GAPDH) (RABBIT) Antibody600-401-A33
Loading Control Antibodies are antibodies that recognize mostly housekeeping proteins in cells used in a scientific experiment. Loading controls are essential for the interpretation of western blot data. Loading control antibodies allow the verification of equal protein loading between samples. Ideal loading controls are expressed constitutively and at high levels with low variability between cell lines and experimental conditions. Also, the loading control should be at a different molecular weight than the protein of interest this allows the bands to be visually distinguishable.
Rockland offers a variety of cell lysates that can be used as controls for Western blot analysis experiments. Lysates are generated either from whole cells which contain cell membrane, cytoplasmic and nuclear proteins, or nuclear extracts, which are predominantly proteins that originate in the nucleus. Control lysates may be from cells that are stimulated with insulin, doxorubicin, etoposide, nocodozole, TNFα or EGF. Lysates are also available from normal animal tissue derived from primary organs such as liver, heart and brain.
Purified proteins or peptides are ideal as controls in FLOW, Western Blot and Elisa. Proteins can be used as loading control on western blot experiments or as titration agents in ELISA experiments. Rockland produces purified immunoglobulin proteins from a variety of species, often available by immunoglobulin class or as fragments of immunoglobulins. Peptides can be used to do competition assays or to be used in peptide arrays. Click here for controls for Host Cell Protein assays.
Low endotoxin control proteins are IgG preparations of whole mouse serum purified by protein A chromatography using a low endotoxin methodology. They are ideal as controls in neutralization experiments, ELISA, FLOW and other assays.
The Mouse TrueBlot® Western Blot Kit contains the critical supporting reagents, buffers, and substrates for GFP immunoprecipitation and Western blotting of samples using TrueBlot second step immunoblotting reagents in conjunction with your own primary IP antibody and primary (Mouse IgG) Western blotting antibody. TrueBlot technology enables unhindered detection of protein bands of interest which would otherwise be obscured by the presence of reduced and denatured heavy and light chain immunoglobulin in the blot (as detected by the conventional immunoblotting HRP anti-mouse IgG reagent).
Rockland Immunochemicals Inc.Gilbertsville, PA 19525E-mail: firstname.lastname@example.orgPhone: 800.656.7625