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        Control Cell Lysates and Nuclear Extracts


Control Cell Lysates and Nuclear Extracts


Rockland offers control cell lysates and nuclear extracts for use on SDS PAGE as standalone samples or in combination with antibodies on Western Blotting experiments. Our ready-to-use whole cell lysates and nuclear extracts are derived from cell lines or tissues using highly advanced extraction protocols to ensure remarkably high quality, protein integrity and lot-to-lot reproducibility.   Control Cell Lysates and Nuclear Extracts  
Lysates are generated either from whole cells which contain cell membrane, cytoplasmic and nuclear proteins, or nuclear extracts, which are predominantly proteins that originate in the nucleus. Control lysates may be from cells that are stimulated with insulin, doxorubicin, etoposide, nocodozole, TNFa or EGF. Lysates are also available from normal animal tissue derived from primary organs such as liver, heart and brain. Further, Rockland also offers a variety of lysates that contain over expressed proteins (tagged and untagged) that can serve as positive controls for antibody reactivity. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.
 

12 Epitope Tag Protein Marker Lysate

Normal Mouse Heart Whole Cell Lysate

HeLa Whole Cell Lysate

12 Epitope Tag
Protein Marker Lysate
MB-301-0100 

Normal Mouse Heart
Whole Cell Lysate
W10-000-T014 

HeLa Whole
Cell Lysate
W09-000-364 

     

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Recommended Control Cell Lysates and Nuclear Extracts:

  • EGF Stimulation controls use A431 Whole Cell Lysate - EGF Stimulated - W09-000-362.
  • Positive control for anti-epitope tag antibodies use 12 Epitope Tag Protein Marker Lysate - MB-301-0100. This E.coli lysate contains a recombinant protein comprised of the following commonly used epitope tags: MBP--T7--HSV--cMyc--VSV--Glu-Glu--V5--E-tag--Flag--S tag--HA--6XHis.
  • For Human Fibroblasts use Human Foreskin Fibroblast Whole Cell Lysate - W09-001-375
  • For Host Cell Protein control (HCP) use E.coli Host Cell Protein Control Protein (HCP) - 000-001-J08

    


 

The Rockland Advantage

Rockland incorporates scientific expertise with customized protocols and proven development methods to provide seamless and reliable control lysates for your applications. Rigorous quality control testing ensures that finished purified products meets or exceeds our high standards for optimum performance in your assays.

 
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