Antibodies - Rockland Immunochemicals primary and secondary antibodies, conjugates and custom antibody production, Custom Monoclonal Antibodies, Custom Polyclonal Antibodies, Custom Molecular Biology, Custom Conjugations, Custom Antibody Purification, Aff

Rockland's custom polyclonal antibody service department has extensive experience in generating high-titer, high-affinity antisera to a wide variety of antigens. Our staff is ready to assist you in experimental design, laboratory methods, production, purification and conjugation. If you have any questions about experimental designs please contact our Custom Services Group via e-mail or by calling them at 1-800-656-ROCK. Below are some Frequently Asked Questions (FAQs).

How much antigen is required? Using proprietary immunization methodologies as little as 200 micrograms of antigen per injection can be used for a standard immunization protocol. However, we have been successful using approximately 50 micrograms per injection for valuable antigens. If antigen is abundant immunize with 0.1 to 0.5 mg per injection.

Rapid Screen (1 month)

Fast Protocol (6 weeks)

Standard (Monthly)

100 mls Yield Per Rabbit Schedule

75 mls Yield per Month per Rabbit Schedule

Day	Activity
-4	Pre-bleed
0	Immunization
7	Booster
14	Booster
28	Booster
38	Terminal Bleed
38	Ship

Day	Activity
-4	Pre-bleed
0	Immunization
7	Booster
14	Booster
28	Booster
38	Test Bleed
38	Ship
45	Terminal Bleed
52	Ship

Day	Activity
-4	Pre-bleed
0	Immunization
7, 14, 28	Booster
38	Test Bleed
45,52	Production bleed
56	Booster
59	Ship
66, 73, 80	Production Bleeds
84	Booster
87	Ship
94+	Repeat Above

How many animals should I put on a study? We recommend two or more animals to allow for immune response variability.

What type(s) of antigens can be injected? We can immunize animals with native proteins, GST-fusion proteins, peptides, carbohydrates or any other compound of viral, fungal or microbial origin. However, no biohazardous material can be accepted. We recommend the use of Freund's Adjuvant, however, we can accommodate any requirements that you may have for specialized adjuvants.

Can you conjugate my peptide to a carrier? Yes. Please consult with our technical staff to recommend peptide synthesis strategies to facilitate carrier coupling and enhance peptide immunogenecity.

How should I send the antigen? Antigen can be supplied in a soluble form, as a lyophilizate, a gel slice or on a nitrocellulose membrane. An additional charge of $35 will be applied for gel slice extraction. Please call prior to shipping so that we can prepare to start your project.

Is Rockland a USDA approved and NIH Licensed facility? Yes. Our staff can assist you in completing necessary forms for NIH. Our in-house IACUC completes and maintains animal protocols eliminating your paperwork load.
USDA #23-R-134
NIH BPA #00008695
NIH Assurance OPRR #A4062-01

We offer polyclonal antibody production in a wide variety of hosts, including Rabbit, Goat, Sheep, Guinea Pig, Chicken, Rat, Mouse, and Donkey.

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Additional Custom Polyclonal Antibody Services
Service	Code	Price
Rapid Screen for Peptides	CUST61	Get a Quote
This service is designed for the rapid screening of peptides usually delivered in about one (1) month! this is the fastest way to get custom polyclonal antibody for your immunoassay. This service includes analysis of the peptide's primary structure, peptide synthesis (up to 15 amino acids), conjugated to KLH carrier protein, pre-bleeding of two (2) rabbits, immunization, booster injections, terminal bleeding of rabbits and titer analysis of the antiserum collected by indirect ELISA. We will provide you with approximately 100ml of antiserum, the pre-immune serum and any remaining unconjugated peptide. Call for more details.
Pre-screening of host animal	CUST37	Get a Quote
Host animals may show elevated titers in pre-immune sera to certain antigens of bacterial or fungal origin. If you are studying bacterial or fungal proteins you may request pre-screening of rabbits at no additional charge Pre-bleed and daily maintenance fees apply to all host animals reserved for your selection. After the host animals are selected the charge for animals would apply only to those host animals that meet your requirements

Inquire for pricing and availability of other host animals
including,. but not limited to, bovine. rat, guinea pig, hamster,
turkey, donkey or swine. All of our customers are ensured
ownership and confidentiality of each project.

We offer polyclonal antibody production in a wide variety of hosts, including Rabbit, Goat, Sheep, Guinea Pig, Chicken, Rat, Mouse, and Donkey.

Get a Quote

Additional Laboratory Services for further processing of antiserum are also available