Antibodies - Rockland Immunochemicals primary and secondary antibodies, conjugates and custom antibody production, Custom Monoclonal Antibodies, Custom Polyclonal Antibodies, Custom Molecular Biology, Custom Conjugations, Custom Antibody Purification, Aff

MAMMALIAN HOST SYSTEM

BACTERIAL HOST SYSTEM
Service	Code	Price
Vector Construction	MBS-001	Get a Quote
Provide the gene insert either purified or within purified plasmid or contained in a slab culture and Rockland will excise the insert and ligate it into an appropriate bacterial expression vector. Transformation of E.coli using subcloned plasmid will be performed. Colonies will be screened using conventional methodologies. Slab cultures require plasmid isolation prior to subcloning.
Computational Analysis	MBS-018	Get a Quote
The latest software is used to analyze your DNA or protein sequence to project the physical charcteristics of the expressed protein, including extinction coefficient, isoelectric point, charge, hydrophobicity and secondary structure. BLAST is performed to compare your sequence to published sequences to determine common and species-specific regions to aid with the design of peptides. A complete restriction site analysis is performed to aid in vector construction. A search for silent mutations that result in the appearance or disappearance of restriction sites without changing the protein sequence is performed. Oligonucleotides for PCR amplification, sequencing primers, and hybidization probes are designed and analyzed for melting temperatures, dimer and hairpin loop formation.
Plasmid Isolation	MBS-002	Get a Quote
Rockland will grow cultures of transformed E.coli and isolate the plasmid from the bacterial host using a mini-prep system. This service can be used in conjunction with subcloning services listed above or an investigator can provide transformed E.coli ready for scale-up and isolation.
Plasmid Restriction Mapping	MBS-003	Get a Quote
A subcloned plasmid is extensively tested using specific restriction endonucleases and agarose gel electrophoresis (AGE) to generate a detailed restriction map of your construct.
Bacterial Protein Expression	MBS-004	Get a Quote
This recombinant protein will be expressed in a bacterial system at the cell culture volume of your choice. Price is based on a 1 liter culture.
Oligonucleotide Synthesis	MBS-019	Get a Quote
Oligonucleotides can be synthesized according to your custom sequence. The Oligos are deprotected, desalted and are supplied in a lyophilized form. Contact Technical Service for additional details.
Nucleotide Sequencing	MBS-020	Get a Quote
Nucleic acids can be fully sequenced according to your specific requirements. Either single strand or double strand analysis of oligos, DNA fragments closed in plasmids, or other forms of DNA can be performed. contact Technical Service for additional details.
BACULOVIRUS HOST SYSTEM
Service	Code	Price
Recombinant Baculovirus Production	MBS-005	Get a Quote
Rockland will use subcloned DNA to produce recombinant baculovirus. The recombinant baculovirus is used to infect SF9 cells or other cells to produce quantities of recombinant baculovirus for subsequent protein expression.
Baculovirus Placque Assay	MBS-006	Get a Quote
Recombinant virus will be used in a placque assay to determine the number of placque forming units (pfu) per ml of viral stock. The PFU value is essential foe the infecton of cells for recombinant protein expression
Recombinant Baculovirus Amplification	MBS-007	Get a Quote
Recombinant Baculovirus will be used to infect SF9 cells to produce quantities of recombinant baculovirus for subsequent protein expression.
Baculovirus MOI Optimization	MBS-008	Get a Quote
Rockland will optimize the multiplicity of infection (MOI) for large scale production of recombinant protein from baculovirus by infecting up to 6 small-scale cultures at varying MOIs and analyzing the expression results by western blot analysis.
Baculovirus Protein Expression	MBS-009	Get a Quote
The recombinant protein will be expressed in a baculovirus system at the cell culture volume of your choice. Price is based on a 1 liter culture.
MAMMALIAN HOST SYSTEM
Service	Code	Price
Mammalian Transient Transfection	MBS-010	Get a Quote
A transient transfection of cells will be performed using either calcium phosphate, electroporation or liposome fusion.
Mammalian Stable Transfection	MBS-011	Get a Quote
A stable transfection of cells will be performed using either calcium phosphate, electroporation or liposome fusion followed by application of antibiotic pressure to up to 5 colonies for the selection of cells that retain multiple copies of expression vector.
Mammalian Retroviral Transfection	MBS-012	Get a Quote
A retroviral vector transfection is suggested for highly efficient mammalian protein expression and for transfections of cell lines that are notoriously difficult to transfect. Retroviral transfection is also suggested when mammalian primary cell lines are derived.
Mammalian Protein Expression	MBS-013	Get a Quote
The recombinant protein will be expressed in a mammalian system using your choice of mammalian cell lines. Typically CHO, 293 or NIH 3T3 cells are used. Price is based on a 1000 cm?of culture.
PURIFICATION OF EXPRESSED PROTEIN
Service	Code	Price
Poly His Tag Protein Purification	MBS-014	Get a Quote
After expression your protein will be thoroughly purified from contaminating proteins using a multi-step process that includes the affinity of polyhistidine for metal ligand chromatogaphy. The purified protein is analyzed by polyacrylamide gel electrophoresis (PAGE). Price is based on purification from 1 liter of culture.
GST Tag Protein Purification	MBS-015	Get a Quote
After expression your protein will be thoroughly purified from contaminating proteins using a multi-step process that includes the affinity of GST for glutathione for affinity chromatography. The purified protein is analyzed by polyacrylamide gel electrophoresis (PAGE). Price is based on purification from 1 liter of culture.
Other Protein Purification	MBS-016	Get a Quote
Rockland will employ a customized methodology to isolate your protein from ant other expression mixture. The specific details of the method will be fully developed and discussed jointly between the scientist and our technical staff. The protein is purified to homogeneity and is suitable use and an immunogen for monoclonal or polyclonal antibody generation.
Other Molecular Biological Services	MBS-017	Get a Quote
Please inquire about nearly any other molecular biology service not listed above. In mostly all instances, custom services in molecular biology are highly specialized to an investigator's particular requirements and may or may not fit well with the listed descriptions of service. Our technical staff welcomes inquires on any related methodology.
DNA Isolation/cDNA Library
Service	Code	Price
Genomic DNA Isolation	MBS-032	Get a Quote
High molecular weight DNA is isolated from either frozen tissue or tissue culture cell preparations. The isolated DNA is tested by restriction enzyme digestion and a photograph is provided for documentation.
cDNA Library	MBS-033	Get a Quote
A cDNA Library is generated in one of several available vectors using as little as 20ug of mRNA. We recommend using the oligo(dT) or a random primer for cDNA synthesis. At your request the library can be further amplified.
Other Molecular Biology Services	MBS-017	Get a Quote
Please inquire about nearly any other molecular biology service not listed above. In mostly all instances, custom services in molecular biology are highly specialized to an investigator's particular requirements and may or may not fit well with the listed descriptions of service. Our technical staff welcomes inquires on any related methodology.