Cell Viability Assay and Antibodies


       

Cell Survival Assays and Antibodies

 

Intracellular ThiolEZ™ Cell Survival Assay

 

Anti-BrdU Antibodies

  

Oxphos™ Cell Survival Assay

  

CellCountEZ™ Cell Survival Assay Kit  

RadChemDosi™ Cell Survival Assay  

 

  

 

 

   
     
             View all BrdU Antibodies                                   View All Cell Viability Antibodies
 
 
 

Cell Survival Assays and Antibodies 

 
 
Cell Viability assays and BrdU detection antibodies are ideal for the determination of proliferation, cell cycle, or cell survival, based on a total cell population. Cell viability assesses healthy cells in a sample, with no distinction between dividing or quiescent cells. An increase in cell viability indicates cell growth, while a decrease in viability can generally be interpreted as the result of either toxic effects of compounds/agents or suboptimal culture conditions.  
 
 
 

Anti-BrdU Antibodies 

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a synthetic thymidine nucleoside analog. BrdU is commonly used to allow the detection of growing or proliferating cells in living tissues.

 

Immunocytochemical detection of BrdU incorporated into DNA is a powerful tool to study cytokinetics of normal neoplastic cells. When cells are cultured with medium containing BrdU, this pyrimidine analog is incorporated in place of thymidine into the replicating DNA of proliferating cells. In vitro or in vivo labeling of tumor cells with BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. The amount of BrdU incorporated into the newly synthesized DNA of S-phase cells is a direct indication of cell proliferation. Also, dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU / DNA FACS / Flow Cytometry analysis.
 
 

 

 

 

 

 

 
 
 
 
 
 
 
View all BrdU Antibodies
 

 

 

 

 

CellCountEZ™ Cell Survival Assay Kit 

The CellCountEZ™ cell survival assay kit offers many advantages that make it superior to common existing methods for quantifying cell growth and survival. This Cell Viability Assay is a tissue culture media-based assay that can measure and is based on the ability of mammalian cells to rapidly and efficiently convert hydroxyethyl disulfide (HEDS) into mercaptoethanol (ME) through a biorreduction mechanism. Bioconversion of HEDS to ME relies on the activity of the oxidative pentose phosphate cycle (OPPC). CellCountEZ™ measures ME in the extracellular medium without the need for cellular lysis and extraction methods. This preserves the ability to perform other cellular tests in the same culture system. CellCountEZ™ is readily soluble, membrane permeable and converted by live cells intracellularly, before transport into the extracellular culture media.
 
  • Consistent results from tests on the same sample
  • Optical Density Range is up to 3.0 per 40,000 cells
  • Consistent reproducibilty of results means the test is more reliable, resulting in a reduced need for follow-on testing
  • CellCountEZ™ and other reagents in kit are stable for at least six months.
  • No mixing with electron acceptor/donor
  • Measured by standard microplate reader
  • Sample reusable when the cells are washed off of the reagents

 

 

   

CellCount EZ™

  

Best of XTT & WST   

(Tetrazolium Salts)  
         

 

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Quantitate Cell Proliferation, Ideal for Drug Screening and Toxicology, Readily Soluable and Membrane Permeable
 

 

 

CellCountEZ Figure

 

 

 

 

Intracellular ThiolEZ™ Cell Survival Assay

  

Glutathione is a natural antioxidant and a major thiol that limits cellular damage in healthy tissues during oxidative stress. It is also centrally involved in repairing damage induced by cancer drugs and radiation and in the detoxification of several commonly used cancer chemotherapeutic drugs. The Intracellular ThiolEZ™ cell survival assay kit measures total intracellular thiols including glutathione and cysteine in a simple and user friendly assay. If may have multiple applications for research in aging, oxidative stress, antioxidant screening, chemotherapy response and toxicology.
 
Contains enough reagents for 100 assays and measure Total Intracellular thiols including glutathione and cysteine.
 
   

ThiolEZ™

  

Best of totalthiol detection

(HPLC, biochemical assays)
         

 

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TotalThiolEZ Figure

 

 

 
 
 

Oxphos™ Cell Survival Assay

 

The Oxphos™ cell survival assay measures the "antioxidant capacity" of cells that requires glutathione. The reagents used are unique in measuring several cytosolic glutathione dependent antioxidant pathways in a single metabolic assay. Oxphos™ offers many advantages that make it superior and unique to existing methods since it is the only assay that quantifies glutathione function / recycling / glucose dependent antioxidant capacity of all mammalian cells and whole blood.  
 
Obtain Linear Response for Mammalian Cells, Antioxidant Screening, Chemotherapy Response and Toxicology. 
 

Oxphos™
   

 

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Oxphos figure

 

 
 
 

RadChemDosi™ Cell Survival Assay  

 

RadChemDosi™ is a chemical based Cell Viability Assay that can measure the dose rate of ionizing radiation. RadChemDosi™ measures the dose rate for ionizing radiation by monitoring ionizing radiation to induce changes in certain chemicals. RadChemDosi™ cell survival assay is ideal for blood irradiators where it can be used for periodic calibration and to determine the dose given to blood by simultaneously irradiating RadChemDosi™ in small disposable tubes outside the bag.
 
 
Chemical Based Viability Assay and sensitive to measure the dose rate as low as 2.5 Gy/min.
 
   

RadChemDosi™

  

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 $3,600

 

 

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TotalThiolEZ Figure

 

 

  

           

 

 

TrueBlot® Mouse

 

Western Blot Kit  

TrueBlot IP/Western Blot Kits Mouse Rabbit Goat

 

 

The Mouse TrueBlot® Western Blot Kit contains the critical supporting reagents, buffers, and substrates for Akt immunoprecipitation and Western blotting of samples using TrueBlot second step immunoblotting reagents in conjunction with your own primary IP antibody and primary (Mouse IgG) Western blotting antibody. TrueBlot technology enables unhindered detection of protein bands of interest which would otherwise be obscured by the presence of reduced and denatured heavy and light chain immunoglobulin in the blot (as detected by the conventional immunoblotting HRP anti-mouse IgG reagent). Mouse IgG TrueBlot® ULTRA is the unique horseradish peroxidase conjugated anti-mouse IgG immunoblotting second step reagent which enables detection of Akt immunoblotted target protein bands, without hindrance by interfering immunoglobulin heavy and light chains from your IP antibody. Use it in place of your usual HRP anti-mouse IgG immunoblotting second step reagent. It is easy to generate publication-quality Akt IP Western Blot data with Mouse IgG TrueBlot® ULTRA.

 

Mouse IgG TrueBlot ULTRA is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of mouse IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Mouse IgG TrueBlot ULTRA with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in Akt IP Western Blotting applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions.

 

IP / Western Blotting comparison of TrueBlot and conventional reagents  


 

 


  

 

New Lab Set Up

 

 

 

     

 

 

FOR LIFE SCIENCE RESEARCH USE ONLY