Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form.
Application Notes:
Suitable for use in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user.
General References:
1. Bertrand FE, Eckfeldt CE, Lysholm AS, LeBien TW. (2000) Notch-1 and Notch-2 exhibit unique patterns of expression in human B-lineage cells. Leukemia. 12: 2095-102.