Skp/Cullin/F-box (SCF) complexes are part of a large family of E3 ubiquitin ligases in which F-box proteins determine substrate specificity. Substrate selection by F-box proteins may also be aided by accessory cofactors.
Application Notes:
Suitable for use in Western Blotting and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user.
General References:
1. Lin DI, Barbash O, Kumar KGS, Weber JD, Harper JW, Klein-Szanto AJP, Rustgi A, Fuchs SY, Diehl JA (2006) Phosphorylation-dependent ubiquitination of cyclin D1 by the SCFFBX4-ĄB crystallin complex. Molecular Cell.
2. Lee TH, Perrem K, Harper JW, Lu KP, Zhou XZ (2006) The F-box protein FBX4 targets PIN2/TRF1 for ubiquitin-mediated degradation and regulates telomere maintenance. J. Biol. Chem. 281(2): 759-768.
3. den Engelsman J, Keijsers V, de Jong WW, Boelens WC (2003) The small heat-shock protein alpha B-crystallin promotes FBX4-dependent ubiquitination. J. Biol. Chem. 278(7): 4699-4704.
4. Chiaur DS, Murthy S, Cenciarelli C, Parks W, Loda M, Inghirami G, Demetrick D, Pagano M (2000) Five human genes encoding F-box proteins: chromosome mapping and analysis in human tumors. Cytogenet. Cell Genet. 88(3-4): 255-258.
