Anti-SARS-CoV Nonstructural Protein 13 (nsp13) (Rabbit)
Host :
Rabbit Anti-SARS-CoV Nonstructural Protein 13 (nsp13)
Conjugation:
Unconjugated
Background Information:
The coronavirus nonstructural protein 13 (nsp13) is one of the SARS-Coronavirus replicase cleaving products encoded by ORF1b. Nsp13 is thought to be part of the viral replication complex, which is associated with intracellular membranes.
Application Notes:
Suitable for use in Western Blotting, Immunofluorescence microscopy and Immunoelectron microscopy. Specific conditions for reactivity should be optimized by the end user.
General References:
1. Snijder, E. J., P. J. Bredenbeek, J. C. Dobbe, V. Thiel, J. Ziebuhr, L. L. M. Poon, Y. Guan, M. Rozanov, W. J. M. Spaan, and A. E. Gorbalenya. 2003. Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage. J. Mol. Bio. 331:991-1004.
2. Prentice, E., J. McAuliffe, X. T. Lu, K. Subbarao, and M. R. Denison. 2004. Identification and characterization of severe acute respiratory syndrome coronavirus replicase proteins. J. Virol. 78:9977-9986.
3. Ivanov, K. A., V. Thiel, J. C. Dobbe, Y. van der Meer, E. J. Snijder, and J. Ziebuhr. 2004. Multiple enzymatic activities associated with Severe acute respiratory syndrome coronavirus helicase. J. Virol. 78:5619-5632.
4. Seybert, A., A. Hegyi, S. G. Siddell, and J. Ziebuhr. 2000. The human coronavirus 229E superfamily 1 helicase has RNA and DNA duplex-unwinding activities with 5'-to-3' polarity. RNA. 6:1056-1068.
5. Snijder, E.J., van der Meer, Y., Zevenhoven-Dobbe, J.C., Onderwater, J.J.M., van der Meulen, J., Koerten, H.K., and Mommaas, A.M. 2006. Ultrastructure and origin of membrane vesicles associated with the SARS-coronavirus replication complex. Manuscript in preparation.
