Anti-SARS-CoV Nonstructural Protein 3 (nsp3) (Rabbit)
Host :
Rabbit Anti-SARS-CoV Nonstructural Protein 3 (nsp3)
Conjugation:
Unconjugated
Background Information:
The nonstructural protein 3 (nsp3) is one of the SARS-Coronavirus replicase cleaving products, encoded by ORF1a. Nsp3 is thought to be part of the viral replication complex, which is associated with intracellular membranes.
Application Notes:
Suitable for use in Immunofluorescence microscopy, immunoelectron microscopy, Immunoprecipitation and by Western Blot. Specific conditions for reactivity should be optimized by the end user.
General References:
1. Snijder, E. J., P. J. Bredenbeek, J. C. Dobbe, V. Thiel, J. Ziebuhr, L. L. M. Poon, Y. Guan, M. Rozanov, W. J. M. Spaan, and A. E. Gorbalenya. 2003. Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage. J. Mol. Bio. 331:991-1004.
2. Prentice, E., J. McAuliffe, X. T. Lu, K. Subbarao, and M. R. Denison. 2004. Identification and characterization of severe acute respiratory syndrome coronavirus replicase proteins. J. Virol. 78:9977-9986.
3. Harcourt, B. H., D. Jukneliene, A. Kanjanahaluethai, J. Bechill, K. M. Severson, C. M. Smith, P. A. Rota, and S. C. Baker. 2004. Identification of severe acute respiratory syndrome coronavirus replicase products and characterization of papain-like protease activity. J. Virol. 78:13600-13612.
4. Putics, A., Filipowicz, W., Hall, J., Gorbalenya, A.E., and Ziebuhr, J. 2005. ADP-ribose-1"-monophosphatase: a conserved coronavirus enzyme that is dispensable for viral replication in tissue culture. J. Virol. 79:12721-12731.
5. Snijder, E.J., van der Meer, Y., Zevenhoven-Dobbe, J.C., Onderwater, J.J.M., van der Meulen, J., Koerten, H.K., and Mommaas, A.M. 2006. Ultrastructure and origin of membrane vesicles associated with the SARS-coronavirus replication complex. Manuscript in preparation.
