CDC20, also known as fizzy, Cell division cycle protein 20 homolog, and p55CDC is required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex.
Application Notes:
This antibody reacts with human CDC20 (fizzy) by Western Blot and Immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates (using HeLa and NIH-3T3, and others).
General References:
1. Jentsch S, Pyrowolakis G. (2000) Ubiquitin and its kin: how close are the family ties? Trends Cell Biol. 10(8):335-42.
2. Song,M.S., Song,S.J., Ayad,N.G., Chang,J.S., Lee,J.H., Hong,H.K., Lee,H., Choi,N., Kim,J., Kim,H., Kim,J.W., Choi,E.J., Kirschner,M.W. and Lim,D.S. (2004) The tumour suppressor RASSF1A regulates mitosis by inhibiting the APC-Cdc20 complex. Nat. Cell Biol. 6 (2), 129-137.
3. Zur,A. and Brandeis,M. (2002) Timing of APC/C substrate degradation is determined by fzy/fzr specificity of destruction boxes. EMBO J. 21 (17), 4500-4510.
4. Asadi,A., Jorgensen,J. and Jacobsson,A. (2002) Elovl1 and p55Cdc genes are localized in a tail-to-tail array and are co-expressed in proliferating cells. J. Biol. Chem. 277 (21), 18494-18500.
5. Cahill,D.P., da Costa,L.T., Carson-Walter,E.B., Kinzler,K.W., Vogelstein,B. and Lengauer,C. (1999) Characterization of MAD2B and other mitotic spindle checkpoint genes. Genomics 58 (2), 181-187.
6. Weinstein,J., Karim,J., Geschwind,D.H., Nelson,S.F., Krumm,J. and Sakamoto,K.M. (1998) Genomic organization, 5' flanking enhancer region, and chromosomal assignment of the cell cycle gene, p55Cdc. Mol. Genet. Metab. 64 (1), 52-57.
7. Weinstein,J. (1997) Cell cycle-regulated expression, phosphorylation, and degradation of p55Cdc. A mammalian homolog of CDC20/Fizzy/slp1. J. Biol. Chem. 272 (45), 28501-28511.
8. Weinstein,J., Jacobsen,F.W., Hsu-Chen,J., Wu,T. and Baum,L.G. (1994) A novel mammalian protein, p55CDC, present in dividing cells is associated with protein kinase activity and has homology to the Saccharomyces cerevisiae cell division cycle proteins Cdc20 and Cdc4. Mol. Cell. Biol. 14 (5), 3350-3363.
