APC11 is also known as Anaphase promoting complex subunit 11, APC11, Cyclosome subunit 11, Hepatocellular carcinoma associated RING finger protein, and HSPC214.
Application Notes:
This antibody reacts with human APC11 by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates (using HeLa and NIH-3T3, and others).
General References:
1. Jentsch S, Pyrowolakis G. (2000) Ubiquitin and its kin: how close are the family ties? Trends Cell Biol. 10(8):335-42.
2. Chan,A.H., Lee,S.M., Chim,S.S., Kok,L.D., Waye,M.M., Lee,C.Y., Fung,K.P. and Tsui,S.K. (2001) Molecular cloning and characterization of a RING-H2 finger protein, ANAPC11, the human homolog of yeast Apc11p. J. Cell. Biochem. 83 (2), 249-258.
3. Gmachl,M., Gieffers,C., Podtelejnikov,A.V., Mann,M. and Peters,J.M. (2000) The RING-H2 finger protein APC11 and the E2 enzyme UBC4 are sufficient to ubiquitinate substrates of the anaphase-promoting complex. Proc. Natl. Acad. Sci. U.S.A. 97 (16), 8973-8978.
4. Tang,Z., Li,B., Bharadwaj,R., Zhu,H., Ozkan,E., Hakala,K., Deisenhofer,J. and Yu,H. (2001) APC2 Cullin protein and APC11 RING protein comprise the minimal ubiquitin ligase module of the anaphase-promoting complex. Mol. Biol. Cell 12 (12), 3839-3851.
Specific References:
1. Ohta T,Michel JJ,Xiong Y, (1999) Association with cullin partners protects ROC proteins from proteasome-dependent degradation.
2. Furukawa M,Ohta T,Xiong Y, (2002) Activation of UBC5 ubiquitin-conjugating enzyme by the RING finger of ROC1 and assembly of active ubiquitin ligases by all cullins. J Biol Chem 277:18, 15758-15765.
