CAND1 is also known as TIP120A, and TATA-binding protein-interacting protein 120A. The SCF complex consists of the invariable components Skp1, Cul1, and Rbx1 as well as a variable F-box protein, and functions as an E3 ubiquitin ligase.
Application Notes:
This antibody reacts with human, rat and mouse CAND1 by western blot and immunoprecipita-tion. The antibody immunoprecipitates in vitro translated protein and protein from cell lysates (using HeLa and NIH-3T3, and others).
General References:
1. Jentsch S, Pyrowolakis G. (2000) Ubiquitin and its kin: how close are the family ties? Trends Cell Biol. 10(8):335-42.
2. Min,K.W., Hwang,J.W., Lee,J.S., Park,Y., Tamura,T.A. and Yoon,J.B. (2003 TIP120A associates with cullins and modulates ubiquitin ligase activity. J. Biol. Chem. 278 (18), 15905-15910.
3. Zheng,J., Yang,X., Harrell,J.M., Ryzhikov,S., Shim,E.H., Lykke-Andersen,K., Wei,N., Sun,H., Kobayashi,R. and Zhang,H. (2002) CAND1 binds to unneddylated CUL1 and regulates the formation of SCF ubiquitin E3 ligase complex. Mol. Cell 10 (6), 1519-1526.
4. Liu,J., Furukawa,M., Matsumoto,T. and Xiong,Y. (2002) NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases. Mol. Cell 10 (6), 1511-1518.
Specific References:
1. Feng S, Shen Y, Sullivan J, Rubio C, Xiong Y (2004) The Arabidopsis thaliana CAND1, an unmodified Cul1 intereacting protein, is involved in multiple devlopmen pathways controlled by ubiquitin/proteasome-mediated protein degradation. Plant Cell in press.
2. Cheng Y, Dai X, Zhao Y. (2004) AtCAND1, a HEAT-repeat protein that participates in auxin signaling in Arabidopsis. Plant Physiol. 135(2):1020-6.
3. Liu J, Furukawa M, Matsumoto T, Xiong Y.(2002) NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases. Mol Cell. 10(6):1511-8.
4. Makino Y, et al.(1999) TATA-Binding protein-interacting protein 120, TIP120, stimulates three classes of eukaryotic transcription via a unique mechanism. Mol Cell Biol. 19(12):7951-60.
5. Makino Y, Yoshida T, Yogosawa S, Tanaka K, Muramatsu M, Tamura TA. (1999) Multiple mammalian proteasomal ATPases, but not proteasome itself, are associated with TATA-binding protein and a novel transcriptional activator, TIP120. Genes Cells. 4(9):529-39.
6. Oshikawa K, Matsumoto M, Yada M, Kamura T, Hatakeyama S, Nakayama KI. Preferential interaction of TIP120A with Cul1 that is not modified by NEDD8 and not associated with Skp1. Biochem Biophys Res Commun. 303(4):1209-16.
7. Zheng J, Yang X, Harrell JM, Ryzhikov S, Shim EH, Lykke-Andersen K, Wei N, Sun H, Kobayashi R, Zhang H. (2002) CAND1 binds to unneddylated CUL1 and regulates the formation of SCF ubiquitin E3 ligase complex. Mol Cell. 10(6):1519-26.
