Cullin 4a is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.
Application Notes:
This antibody reacts with human Cul4A by Western Blot and Immunoprecipitation. The antibody immunoprecipitates protein from cell lysates (using HeLa, U2OS and others). This antibody also co-immunoprecipitates at least 8 subunits of COP9, DDB1 and CAND1.
General References:
1. Jentsch S, Pyrowolakis G. (2000) Ubiquitin and its kin: how close are the family ties? Trends Cell Biol. 10(8):335-42.
2. Chen L.-C., Manjeshwar S., Lu Y., Moore D., Ljung B.M., Kuo W.L., Dairkee S.H., Wernick M., Collins C., Smith H.S.(1998) The human homologue for the Caenorhabditis elegans cul-4 gene is amplified and overexpressed in primary breast cancers. Cancer Res. 58:3677-3683.
Specific References:
1. Higa,L.A., Mihaylov,I.S., Banks,D.P., Zheng,J. and Zhang,H. (2003) Radiation-mediated proteolysis of CDT1 by CUL4-ROC1 and CSN complexes constitutes a new checkpoint. Nat. Cell Biol. 5 (11), 1008-1015.
2. Kipreos,E.T., Lander,L.E., Wing,J.P., He,W.W. and Hedgecock,E.M. (1996) cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family. Cell 85 (6), 829-839.
3. Michel,J.J. and Xiong,Y. (1998) Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A. Cell Growth Differ. 9 (6), 435-449.
4. Ohta,T., Michel,J.J., Schottelius,A.J. and Xiong,Y. (1999) ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Mol. Cell 3 (4), 535-541
5. Mathias, N., Johnson, S.L., Winey, M., Adams, A.E., Goetsch, L., Pringle, J.R., Byers, B., Goebl, M.G. (1996) Cdc53p acts in concert with Cdc4p and Cdc34p to control the G1-to-S-phase transition and identifies a conserved family of proteins. Mol Cell Biol 16:12, 6634-6643.
6. Agnihotri, R., Crawford H.C., Haro, H., Matrisian, L.M., Havrda, M.C. and Liaw, L. (2001) J. Biol. Chem. 276, 28261-28267.
7. Li B, Ruiz JC, Chun KT. (2002) CUL-4A is critical for early embryonic development. Mol Cell Biol. 22(14):4997-5005.
8. Shiyanov P, Nag A, Raychaudhuri P. (1999) Cullin 4A associates with the UV-damaged DNA-binding protein DDB.
