Cullin 2 is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.
Application Notes:
his antibody reacts with human Cul2 by Western Blot and Immunoprecipitation. The antibody immunoprecipitates in vitro translated product and protein from cell lysates (using HeLa or NIH-3T3).
General References:
1. Kamura,T., Sato,S., Iwai,K., Czyzyk-Krzeska,M., Conaway,R.C. and Conaway,J.W. (2000) Activation of HIF1alpha ubiquitination by a reconstituted von Hippel-Lindau (VHL) tumor suppressor complex. Proc. Natl. Acad. Sci. U.S.A. 97 (19), 10430-10435.
2. Wada,H., Yeh,E.T. and Kamitani,T. (1999) Identification of NEDD8-conjugation site in human cullin-2. Biochem. Biophys. Res. Commun. 257 (1), 100-105.
3. Pause,A., Lee,S., Worrell,R.A., Chen,D.Y., Burgess,W.H., Linehan,W.M. and Klausner,R.D. (1997) The von Hippel-Lindau tumor-suppressor gene product forms a stable complex with human CUL-2, a member of the Cdc53 family of proteins. Proc. Natl. Acad. Sci. U.S.A. 94 (6), 2156-2161.
4. Jentsch S, Pyrowolakis G. (2000) Ubiquitin and its kin: how close are the family ties? Trends Cell Biol. 10(8):335-42.
Specific References:
1. Feng, H., Zhong, W., Punkosdy, G., Gu, S., Zhou, L., Seabolt,. E.K. and Kipreos, E.T. (1999) CUL-2 is required for the G1-to S-phase transition and mitotic chromosome condensation in Caenorhabditis elegans. Nat Cell Bio 1, 486-492.
2. Kipreos,E.T., Lander,L.E., Wing,J.P., He,W.W. and Hedgecock,E.M. (1996) cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family. Cell 85 (6), 829-839.
3. Michel,J.J. and Xiong,Y. (1998) Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A. Cell Growth Differ. 9 (6), 435-449.
4. Ohta,T., Michel,J.J., Schottelius,A.J. and Xiong,Y. (1999) ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Mol. Cell 3 (4), 535-541.
5. Mathias, N., Johnson, S.L., Winey, M., Adams, A.E., Goetsch, L., Pringle, J.R., Byers, B., Goebl, M.G. (1996) Cdc53p acts in concert with Cdc4p and Cdc34p to control the G1-to-S-phase transition and identifies a conserved family of proteins. Mol Cell Biol 16:12, 6634-6643.
