Cullin 1 is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.
Application Notes:
This antibody reacts with human Cul1 by Western Blot and Immunoprecipitation. The antibody immunoprecipitates in vitro translated product and protein from cell lysates (using HeLa or NIH-3T3).
General References:
1. Hori,T., Osaka,F., Chiba,T., Miyamoto,C., Okabayashi,K., Shimbara,N., Kato,S. and Tanaka,K. (1999) Covalent modification of all members of human cullin family proteins by NEDD8. Oncogene 18 (48), 6829-6834.
2. Read,M.A., Brownell,J.E., Gladysheva,T.B., Hottelet,M., Parent,L.A., Coggins,M.B., Pierce,J.W., Podust,V.N., Luo,R.-S., Chau,V. and Palombella,V.J. (2000) Nedd8 modification of cul-1 activates SCF(beta(TrCP))-dependent ubiquitination of IkappaBalpha. Mol. Cell. Biol. 20 (7), 2326-2333.
3. Tan,P., Fuchs,S.Y., Chen,A., Wu,K., Gomez,C., Ronai,Z. and Pan,Z.Q. (1999) Recruitment of a ROC1-CUL1 ubiquitin ligase by Skp1 and HOS to catalyze the ubiquitination of I kappa B alpha. Mol. Cell 3 (4), 527-533 (1999).
4. Jentsch S, Pyrowolakis G. (2000) Ubiquitin and its kin: how close are the family ties? Trends Cell Biol. 10(8):335-42.
Specific References:
1. Ohta,T., Michel,J.J., Schottelius,A.J. and Xiong,Y. (1999) ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Mol. Cell 3 (4), 535-541.
2. Michel,J.J. and Xiong,Y. (1998) Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A. Cell Growth Differ. 9 (6), 435-449.
3. Kipreos,E.T., Lander,L.E., Wing,J.P., He,W.W. and Hedgecock,E.M. (1996) cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family. Cell 85 (6), 829-839.
4. Mathias, N., Johnson, S.L., Winey, M., Adams, A.E., Goetsch, L., Pringle, J.R., Byers, B., Goebl, M.G. (1996) Cdc53p acts in concert with Cdc4p and Cdc34p to control the G1-to-S-phase transition and identifies a conserved family of proteins. Mol Cell Biol 16:12, 6634-6643.
