Human IgA (alpha chain) Antibody DyLight™800 Conjugated

Datasheet

Human IgA (alpha chain) Antibody DyLight™800 Conjugated

Name: Human IgA (alpha chain) Antibody DyLight™800 Conjugated
Brand: Rockland Immunochemicals
Price: 111.0 USD
Availability: InStock

Goat Polyclonal

1 References

Item No. 609-145-006

Size: 100 µg

Applications: Dot Blot, Microarray

Reactivity: Human

Host Species: Goat

$111.00 /Per Item

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Background

Anti-Human IgA DyLight 800 Antibody generated in goat detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Expand Background

Specifications for Human IgA (alpha chain) Antibody DyLight™800 Conjugated

Product Details

Description: Goat Anti-Human IgA (alpha chain) Antibody DyLight™800 Conjugated - 609-145-006

Synonyms: Goat Anti Human IgA (alpha chain) Antibody DyLight 800™Conjugated, Goat Anti-Human IgA (alpha chain) Antibody DyLight 800™Conjugated, Goat Anti Human IgA Antibody DyLight 800™Conjugated

Host Species: Goat

Specificity: IgA

Conjugate: DyLight™ 800

Clonality: Polyclonal

Format: IgG

Target Details

Reactivity: Human

Immunogen: Human IgA alpha heavy chain

Purity/Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgA and Human Serum. No reaction was observed against Human IgM or Human IgG. ELISA was used to confirm specificity minimal cross reactivity against other human heavy or light chain isotypes.

Application Details

Tested Applications: Dot Blot

Suggested Applications: Microarray - View References

Application Note: Anti-Human IgA DyLight 800 Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Formulation

Physical State: Lyophilized

Concentration: 1.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Reconstitution Volume: 100 µL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

References (1)

Fathi, A et al. (2022). Increased neutralization and IgG epitope identification after MVA-MERS-S booster vaccination against Middle East respiratory syndrome. Nature Communications

Applications

Peptide Microarray

PubMed

Related Protocols

Adherent Cell Lysis Protocol

General Immunoblotting Protocol

Suspension Cultured Cell Lysis Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.