SDS-PAGE of Mouse CD40 Ligand Recombinant Protein

Datasheet

rMouse CD40 Ligand Protein

Name: rMouse CD40 Ligand Protein
Brand: Rockland Immunochemicals
Availability: OutOfStock

Item No. 010-001-U68-0005

Size: 5 µg

Origin: Mouse

Expressed in: E. coli

This product is discontinued

Background

CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40-L stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40-L has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. CD40-L also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-γ, TNF-α, and IL-2. Recombinant mouse CD40 Ligand is a non-glycosylated protein, containing 149 amino acids and having a molecular mass of 16.4 kDa.

Expand Background

Specifications for rMouse CD40 Ligand Protein

Product Details

Description: Mouse CD40 Ligand Recombinant Protein - 010-001-U68-0005

Synonyms: TNFSF5, TNF-related activation protein (TRAP), CD154, gp39, T-BAM

Species of Origin: Mouse

Expressed in: E. coli

Type: Recombinant Protein

Low Endotoxin:

Target Details

Gene Name: Cd40lg - View All Cd40lg Products

Purity/Specificity: CD40 Ligand purity was determined to be greater than 98% as determined by analysis by HpLC, UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Database Links:

UniProtKB - P27548

Application Details

Tested Applications: SDS-PAGE

Suggested Applications: Cellular Assay - View References

Application Note: CD40 Ligand Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-CD40 Ligand in immunological assays. Buffer Formulation: 10 mM sodium phosphate, 50 mM arginine, pH7.5.

Formulation

Physical State: Lyophilized

Buffer: See application note.

Preservative: None

Stabilizer: None

Reconstitution Volume: 5µl (5-50µl)

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiration: Expiration date is six (6) months from date of receipt.

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.