Datasheet

HO1 protein-GST fusion

Name: HO1 protein-GST fusion
Brand: Rockland Immunochemicals
Price: 325.0 USD
Availability: OutOfStock

Item No. 009-001-S03

Size: 50 µg

Origin: Human

Expressed in: E. coli

$665.00 /Per Item

20 µg $325.00

50 µg $665.00

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Background

HO1 (Heme oxygenase 1) catalyzes the oxidative cleavage of heme to biliverdin and is one of the main genes controlling heme synthesis and catabolism. HO1 plays a protective role in various disorders and can ameliorate experimental MN via multiple pathways, including anti-oxidative and immunomodulatory effects (1). Exposure of primary hepatocytes to carbon monoxide and nitric oxide results in dramatic induction of HO1 in dose- and time-dependent manner and this induction is blocked by MAP kinase inhibitors (MAPKs) but not by inhibitors of PI3 kinase pathway (2). HO1 Protein is ideal for investigators involved in Signaling Proteins, Cell Stress & Chaperone Proteins, Cancer, Cardiovascular Disease, Cellular Stress, Inflammation, and Neurobiology research.

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Specifications for HO1 protein-GST fusion

Product Details

Description: HO1 recombinant protein-GST fusion protein - 009-001-S03

Synonyms: Heme Oxygenase (Decycling) 1, HMOX1, HSP32, bK286B10

Species of Origin: Human

Expressed in: E. coli

Type: Recombinant Protein

Target Details

Gene Name: HMOX1

Purity/Specificity: Recombinant full-length human HO1 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.

Database Links:

NCBI - NM_002133

Application Details

Application Note: HO1 Protein is stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. HO1 Protein is suitable for use in Western Blot. Expect a band approximately ~56kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Formulation

Physical State: Liquid (sterile filtered)

Concentration: 0.2 µg/µL by UV absorbance at 280 nm

Buffer: See application note.

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiration: Expiration date is one (1) year from date of receipt.

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.