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Datasheet

CRYAA protein-HIS Epitope

009-001-R75S 009-001-R75
20 µg 50 µg
Human
E. coli
$189.00 /Per Item
$279.00 /Per Item
Availability: Ships in approximately 30 days Availability: Ships in approximately 30 days
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Background

CRYAA or crystallin alpha A is the major protein components of the vertebrate eye lens which is composed of 2 primary gene products--alpha-A and alpha-B. CRYAA can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family (1). CRYAA plays an important role in the normal embryologic development of the anterior segment of the eye. In the Elo mouse, a 1-bp deletion in the gamma-E-crystallin gene causes autosomal dominant cataract and microphthalmia (2). CRYAA Protein is ideal for investigators involved in Signaling Proteins, Cell Stress & Chaperone Proteins, Cancer, Metabolic Disorder, and Neurobiology research.

Product Details

CRYAA recombinant protein-HIS Epitope - 009-001-R75
CRYA1, HSPB4, Alpha-crystallin A chain, Heat shock protein beta-4, HspB4
Human
E. coli
Recombinant Protein

Target Details

CRYAA
Recombinant full length human CRYAA was expressed in E. coli cells using an N-Terminal his epitope. The purity was determined to be >95% by densitometry.

Application Details

CRYAA Protein is stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol. CRYAA Protein is suitable for use in Western Blot. Expect a band approximately ~ 21kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Formulation

Liquid (sterile filtered)
0.2 µg/µL
See application note.

Shipping & Handling

Dry Ice
Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.