Western Blot of Anti-Dopamine ß-Hydroxylase (Sheep) Antibody - 600-601-D27

Datasheet

Dopamine Beta-Hydroxylase Antibody

Name: Dopamine Beta-Hydroxylase Antibody
Brand: Rockland Immunochemicals
Price: 475.0 USD
Availability: OutOfStock

Sheep Polyclonal

Item No. 600-601-D27

Size: 100 µL

Applications: WB

Reactivity: Human, Mouse, Non-Human Primates

Host Species: Sheep

$475.00 /Per Item

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Background

Dopamine beta Hydroxylase Antibody detects DBH which catalyzes the conversion of dopamine to norepinephrine and serves as a marker of noradrenergic cells. DBH antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse. The expression of DBH is also elevated during stress. Anti-Dopamine beta Hydroxylase Antibody is ideal for investigators involved in Cell Signaling and Neuroscience.

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Specifications for Dopamine Beta-Hydroxylase Antibody

Product Details

Description: Anti-Dopamine ß-Hydroxylase (SHEEP) Antibody - 600-601-D27

Synonyms: Dopamine beta-monooxygenase, DBH

Host Species: Sheep

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: DBH - View All DBH Products

Reactivity: Human, Mouse, Non-Human Primates

Immunogen Type: Conjugated Peptide

Immunogen: Anti-Dopamine beta Hydroxylase Antibody was produced in sheep by repeated immunizations with synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH.

Purity/Specificity: Anti-Dopamine beta Hydroxylase antibody is directed against human dopamine beta hydroxylase. Anti-Dopamine beta Hydroxylase antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Anti-Dopamine beta Hydroxylase antibody recognizes Dopamine beta Hydroxylase from human, mouse, and non-human primate sources. Cross reactivity with Dopamine beta Hydroxylase from other species has not been determined.

Database Links:

UniProtKB - P09172

GeneID - 1621

UniProtKB - P09172.3

Application Details

Tested Applications: WB

Application Note: Anti-Dopamine beta-Hydroxylase Antibody is tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 75 kDa in size corresponding to DBH protein in the appropriate cell lysate or extract.

Formulation

Physical State: Liquid

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Related Protocols

Adherent Cell Lysis Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.