Western Blot of Anti-DARPP-32 pT75 (Rabbit) Antibody - 612-401-D25

Datasheet

DARPP-32 phospho T75 Antibody

Name: DARPP-32 phospho T75 Antibody
Brand: Rockland Immunochemicals
Price: 475.0 USD
Availability: OutOfStock

Rabbit Polyclonal

Item No. 612-401-D25

Size: 100 µL

Applications: WB

Reactivity: Mouse, Rat

Host Species: Rabbit

$475.00 /Per Item

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Background

DARPP-32 antibody detects DARPP-32 which is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons. The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac®. Anti-DARPP-32 pT75 Antibody is ideal for investigators involved in Neuroscience and Cell Signaling Research.

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Specifications for DARPP-32 phospho T75 Antibody

Product Details

Description: Anti-DARPP-32 pT75 (RABBIT) Antibody - 612-401-D25

Synonyms: Protein phosphatase 1 regulatory subunit 1B, Dopamine- and cAMP-regulated neuronal phosphoprotein

Host Species: Rabbit

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: Ppp1r1b - View All Ppp1r1b Products

Reactivity: Mouse, Rat

PTM Specificity: Phosphorylation

Immunogen Type: Conjugated Peptide

Immunogen: Anti-DARPP-32 pT75 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Thr75 conjugated to KLH.

Purity/Specificity: Anti-DARPP-32 pT75 antibody detects rat DARPP-32. Anti-DARPP-32 pT75 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated DARPP-32 pT75 . Minimal reactivity occurs against non-phosphorylated DARPP-32 pT75 . Reactivity is completely blocked by treatment with lambda-phosphatase. Reactivity against DARPP-32 pT75 occurs from rat sources. However, reactivity is also expected against bovine, canine, human, mouse and NHP sources.

Database Links:

UniProtKB - Q6J4I0

GeneID - 360616

UniProtKB - Q6J4I0.1

Application Details

Tested Applications: WB

Application Note: Anti-DARPP-32 pT75 Antibody is tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 32 kDa in size corresponding to DARPP 32 protein phosphorylated at Thr75 in the appropriate cell lysate or extract.

Formulation

Physical State: Liquid

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Related Protocols

Adherent Cell Lysis Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.