Anti-CREB pS133 Antibody - Immunohistochemistry

Datasheet

CREB phospho S133 Antibody

Name: CREB phospho S133 Antibody
Brand: Rockland Immunochemicals
Price: 475.0 USD
Availability: InStock

Rabbit Polyclonal

3 References

Item No. 600-401-270

Size: 100 µL

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Host Species: Rabbit

$475.00 /Per Item

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Background

The CREB (Cyclic AMP-response-element-binding-protein 1) gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element (CRE element TGANNTCA), an octameric palindrome. Phosphorylation by cAMP-dependent protein kinase (PKA) at serine-119 is required for interaction with DNA and phosphorylation at serine-133 allows CREB to interact with CBP (CREB binding protein) leading to interaction with RNA polymerase II. Alternate splicing of this gene results in two transcript variants encoding different isoforms.

Expand Background

Specifications for CREB phospho S133 Antibody

Product Details

Description: Anti-CREB pS133 (RABBIT) Antibody - 600-401-270

Synonyms: rabbit anti-CREB pS133 Antibody, Cyclic AMP-responsive element-binding protein 1, Active transcription factor CREB antibody, cAMP responsive element binding protein 1 antibody, CREB 1 antibody, CREB-1, CREB1 antibody, MGC9284 antibody, Transactivator protein antibody

Host Species: Rabbit

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: CREB1 - View All CREB1 Products

Reactivity: Human, Mouse, Rat

PTM Specificity: Phosphorylation

Immunogen Type: Conjugated Peptide

Immunogen: CREB phospho peptide corresponding to amino acid residues 122-147 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Purity/Specificity: Anti-CREB pS133 was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first sequentially pre-adsorbed against a control E.coli lysate, nickel-purified recombinant CREB and the non-phosphorylated form of the immunizing peptide. The resultant depleted antiserum was then purified against the phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody is specific for phosphorylated pS133 of human CREB. Reactivity with non-phosphorylated CREB is minimal. This antibody does show cross reactivity with pS133 phosphorylated CREB from mouse and rat.

Database Links:

NCBI - CAG28545.1

UniProtKB - P16220

GeneID - 1385

Application Details

Tested Applications: ELISA, WB

Application Note: Anti-CREB pS133 antibody reacts with phosphorylated human CREB at pS133 and shows minimal reactivity by western blot with non-phosphorylated CREB and minimal reactivity (1%) by ELISA against the non-phosphorylated form of the immunizing peptide. This antibody was assayed against a variety of tissues including fibroblasts and B-cell lysates. Bands of 46 and 43 kDa corresponding to phosphorylated CREB are observed in western blots. Anti-CREB pS133 is suitable for the detection by immunoblot of phosphorylated human, mouse and rat CREB. No cross-reactivity occurs with non-phosphorylated CREB. For immunohistochemistry, formalin fixed, paraffin embedded human tissue shows moderate to strong nuclear staining in a variety of cells with minimal background staining. Although not tested this antibody is likely functional by FACS and immunoprecipitation.

Formulation

Physical State: Liquid (sterile filtered)

Concentration: 0.064 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

References (3)

Perez-Schindler J et al. (2011). Regulation of contractility and metabolic signaling by the β2-adrenergic receptor in rat ventricular muscle. Life Sci.

Applications

WB, IB, PCA

PubMed

Philp A, Chen A, Lan D, et al. (2011). Sirtuin 1 (SIRT1) deacetylase activity is not required for mitochondrial biogenesis or peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) deacetylation following endurance exercise. J Biol Chem.

Applications

WB, IB, PCA

PubMed

Reiners J et al. (2007). A polymorphism linked to bipolar affective disorder does not alter the CRE activity of constitutively activated trace amine receptor 4. Mol Psychiatry.

Applications

WB, IB, PCA

PubMed

Related Protocols

Adherent Cell Lysis Protocol

ELISA Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Sandwich ELISA Protocol for Collagen

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.