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        Bio-Rad Collaboration


Rockland and Bio-Rad Collaborate on Enhanced Multiplex Western Blotting: Less Hassle. Better Resolution.

 

Rockland Immunochemicals has collaborated with Bio-Rad to develop an enhanced multiplex fluorescent western blotting protocol. The assays produce high resolution immunoblots on PVDF membrane, providing increased visibility and profiling of low abundance proteins.

 

Rockland DyLight™ fluorochrome conjugated secondary antibodies are used to probe the membrane and the Molecular Imager® VersaDoc™ MP 4000 and the ChemiDoc™ XRS+ imaging system developed by Bio-Rad Laboratories, provides clear, accurate results. Several targets can be detected and imaged simultaneously, resulting in blots that show enhanced detection of distinct target bands labeled by a unique identifying color. Simultaneous detection helps cut time, while ensuring the target epitopes are still easily identified.

 

 

Featured Products:


DyLight Conjugated Rabbit Antibody

 

Anti-RABBIT IgG (H&L) (GOAT) Antibody DyLight™ 488 Conjugated
IRDye 700DX Conjugated Mouse Antibody

 

Anti-MOUSE IgG (H&L) (RABBIT) Antibody IRDye® 700DX Conjugated
GAPDH Rabbit Antibody

 

Anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (RABBIT) Antibody
Alpha Tubulin Mouse Antibody

 

Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody
Blocking Buffers and Water

 

Blocking Buffers

    


  

 
Enhanced HCP ELISA Detection Using Antibodies Validated by Two-Dimensional (2D) Electrophoresis and Western Blotting

 

 

Rockland also collaborated with Bio-Rad on a two-dimensional workflow validation to develop an improved standardized immunoassay for HCP detection. This collaboration resulted in a method using a robust 2D SDS-PAGE using the PROTEAN® i12™ IEF System, ReadyStrip™ IPG Strips, and western blotting assay to validate the efficacy of immunocoverage for novel anti-HCP polyclonal antibodies. Validation is performed using BioRad’s PDQuest 2-D Analysis Software.

 

Rockland offers KCAJ07, an E.coli HCP Western Blot kit that contains two-dimensional validated antibodies, HRP conjugated secondary antibodies, blocking and wash solutions and E.coli control proteins. Rockland’s two-dimensional validated antibodies have been shown to react with more than 300 E.coli proteins from SDS/DTT solubilized E.coli cells.

 


 

Featured Products:


Host Cell Protein Antibody

 

Anti-Host Cell Protein (HCP) Antibody
CHP Control Proteins

 

E.coli Host Cell Protein Control Protein (HCP)
IgG Antibody Peroxidase Conjugated

 

Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated
Chemiluminescent FemtoMax Substrate

 

Chemiluminescent FemtoMax™ Super Sensitive HRP Substrate for Microwell and/or Membrane (2 component system)

 

 

To learn more about HCP antibodies and these new systems or obtain a custom quote, contact Rockland today for information.

  Enhanced Multiplex Western Blot Poster 

Download the Rockland and Bio-Rad Enhanced Multiplex Western Blot Poster



HCP Development Poster 

Download the Rockland and Bio-Rad HCP Development Blot Poster



Bio-Rad Collaboration Videos:


Performance of a Western Blot:


View Performance of a Western Blot video


ChemiDoc™ MP Introduction:


View ChemiDoc™ MP video