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Although we recommend that each investigator determine the actual optimum dilution for a given antibody or antibody conjugate for each specific assay, the following chart can be used as a guideline for initial titration experiments. Enzyme conjugates of Affinity Purified antibodies and primary antibodies are supplied with lot-specific recommended dilutions.
ELISA
Blotting
Flow Cytometry
Immunohistochemistry
nd
50 - 200
200 - 1,000
100 - 200
400
100 - 1,000
5,000 - 20,000
3,000 - 10,000
4,000 - 10,000
2,000 - 5,000
200 - 2,000
1,000 - 2,000
500 - 1,000