TrueBlot® is a secondary antibody which enables unhindered detection of molecules. Simply substitute your conventional HRP or fluorescent reagent with mouse, rabbit, goat or sheep TrueBlot for unparalleled clarity in results and high quality imaging suitable for reproduction.
Immunoprecipitation protocols and Western Blots provide highly specific results, yet often suffer from heavy/light chain blotting, contamination, and ongoing interference. TrueBlot® products solve nearly all of these problems through increased sensitivity, less background noise, and enhanced accuracy.
TrueBlot® reagents enable you to generate clear, best-quality data in individual Immunoprecipitation and Western Blots (Nfkb Western Blots as well as GFP Immunoprecipitation Western Blots), but can also be used when the two protocols are combined. Available in several options, from IP Agarose Beads alone, to complete IP/Western Blot kits from goat, mouse, rabbit or sheep.
Sheep TrueBlot®: Anti-Sheep IgG HRP
TrueBlot® IP and Western Blot Kit for Epitope TagsThe TrueBlot® Immunoprecipitation and Western Blot Kit for Epitope Tags contain the critical supporting reagents, buffers, and substrates for IP and WB of the appropriate tagged proteins. Using TrueBlot will allow easy enrichment and clear monitoring of proteins.
TrueBlot® IP and Western Blot Kit for DYKDDDDK (FLAG®) Epitope Tag
TrueBlot® IP and Western Blot Kit for 6X HIS Epitope Tag
TrueBlot® IP and Western Blot Kit for GFP Epitope Tag
TrueBlot® Magnetic Beads are uniform, non-aggregating, super-paramagnetic beads consisting of a ferric oxide core, functionalized with various silane groups to which the affinity groups (e.g. protein A, protein G, anti-mouse IgG, Nickel and others) are firmly attached. The beads are specifically designed, tested and quality controlled for sample preparation, or capturing and purifying targets such as proteins, antigens, antibodies, DNA/RNA, cells and more. TrueBlot® Magnetic Bead design enables faster binding kinetics with high sensitivity and selectivity in both manual and automated biomedical and research applications.
Rockland’s TrueBlot® product line enables researchers to generate publication-quality western blot data for Nfkb IP Western Blots, Phospho IP Western Blots, and GFP IP Western Blots. Simply substitute your conventional HRP blotting reagent with Goat TrueBlot®, Mouse TrueBlot®, Rabbit TrueBlot® or Sheep TrueBlot®. TrueBlot® is a horseradish peroxidase conjugated immunoblotting detection reagent, which enables unhindered detection of blotted target bands. This results in unparalleled clarity in results and high quality imaging suitable for reproduction.
TrueBlot® preferentially detects the native disulfide form of mouse IgG or rabbit IgG, making it ideal for IP and Western Blot protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot® reduces interference by the ~55 kDa heavy and ~23 kDa light chains of the immunoprecipitating antibody in IP/immunoblotting applications, making Phospho Immunoprecipitation Western Blot data even more accurate. TrueBlot® is also ideal for studying post-translational protein modifications, including phosphorylation, or protein-protein interactions.
For more information or to request a quote for a specific application, contact us today.
FLAG™ is a registered trademark of Sigma-Aldrich Co. LLC
IP / Western Blot Protocol TrueBlot®
Rockland Immunochemicals Inc.Limerick, PA 19468E-mail: email@example.comPhone: 800.656.7625