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Immunoprecipitation protocols and Western Blots provide highly specific results, yet often suffer from heavy/light chain blotting, contamination, and ongoing interference. TrueBlot® products solve nearly all of these problems through increased sensitivity, less background noise, and enhanced accuracy.
TrueBlot® reagents enable you to generate clear, best-quality data in individual Immunoprecipitation and Western Blots (Nfkb Western Blots as well as GFP Immunoprecipitation Western Blots), but can also be used when the two protocols are combined. Available in several options, from IP Beads alone, to complete IP/Western Blot kits from goat, mouse, rabbit or sheep.
Sheep TrueBlot®: Anti-Sheep IgG HRP
TrueBlot® IP and Western Blot Kit for
DYKDDDDK (FLAG®) Epitope Tag
The TrueBlot® Immunoprecipitation and Western Blot Kit for DYKDDDDK (FLAG®) Epitope Tag contains the critical supporting reagents, buffers, and substrates for IP and WB of DYKDDDDK (FLAG™) tagged proteins. Using TrueBlot will allow easy enrichment and clear monitoring of FLAG™ tagged proteins.
View all Fluorescent Trueblot products
Rockland is proud to present a new family of products to our customers. Fluorescent TrueBlot®, a new format of our popular TrueBlot® product line, combines the power and specificity of the original products with the versatility of fluorescent and near infra-red dyes. Conjugating highly optimized TrueBlot® reagents to a full spectrum of fluorescent labels, results in multipurpose reporter molecules that can be used in a variety of immunoassays.
Rockland’s TrueBlot® product line enables researchers to generate publication-quality western blot data for Nfkb IP Western Blots, Phospho IP Western Blots, and GFP IP Western Blots. Simply substitute your conventional HRP blotting reagent with Goat TrueBlot®, Mouse TrueBlot®, Rabbit TrueBlot® or Sheep TrueBlot®. TrueBlot® is a horseradish peroxidase conjugated immunoblotting detection reagent, which enables unhindered detection of blotted target bands. This results in unparalleled clarity in results and high quality imaging suitable for reproduction.
TrueBlot® preferentially detects the native disulfide form of mouse IgG or rabbit IgG, making it ideal for IP and Western Blot protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot® reduces interference by the ~55 kDa heavy and ~23 kDa light chains of the immunoprecipitating antibody in IP/immunoblotting applications, making Phospho Immunoprecipitation Western Blot data even more accurate. TrueBlot® is also ideal for studying post-translational protein modifications, including phosphorylation, or protein-protein interactions.
For more information or to request a quote for a specific application, contact us today.
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IP Western Blotting Reagents PDF
IP / Western Blot Protocol TrueBlot®
Rockland Immunochemicals Inc.Limerick, PA 19468E-mail: email@example.comPhone: 800.656.7625