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IP Western blots provide highly specific results, yet often suffer from heavy/light chain blotting, contamination, and ongoing interference. TrueBlot® products solve nearly all of these problems through increased sensitivity, less background noise, and enhanced accuracy.
TrueBlot® reagents enable you to generate clear, best-quality data in your Immunoprecipitation and Western Blot protocols. Available in several options, from IP Beads alone, to complete IP/Western Blot kits from goat, mouse, rabbit or sheep.
Rockland’s TrueBlot® product line enables researchers to generate publication-quality IP western blot data. Simply substitute your conventional HRP blotting reagent with Goat TrueBlot®, Mouse TrueBlot®, Rabbit TrueBlot® or Sheep TrueBlot®. TrueBlot® is a horseradish peroxidase conjugated immunoblotting detection reagent, which enables unhindered detection of blotted target bands. This results in unparalleled clarity in results and high quality imaging suitable for reproduction.
TrueBlot® preferentially detects the native disulfide form of mouse IgG or rabbit IgG, making it ideal for IP western blot protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot® reduces interference by the ~55 kDa heavy and ~23 kDa light chains of the immunoprecipitating antibody in IP/immunoblotting applications. TrueBlot® is also ideal for studying post-translational protein modifications, including phosphorylation, or protein-protein interactions.
For more information or to request a quote for a specific application, contact us today.
The TrueBlot® Concept Map outlines the major mechanisms by which TrueBlot® can create exceptionally clear results.
Read more about TrueBlot® Publication-quality IP western blot data.
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IP / Western Blot Protocol TrueBlot®