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TrueBlot® reagents allow the generation of publication-quality data in your Immunoprecipitation, Western Blot and IP Western Blot (GFP IP Western Blot, GFP IP Western Blot, and Phospho IP Western Blot) protocols. Available for goat, mouse, rabbit and sheep primary antibodies, TrueBlot® preferentially detects the native, disulfide form of IgG, effectively eliminating the appearance of non-specific, immunoglobulin bands from your immunoprecipitating antibody, in SDS-PAGE. Use TrueBlot® to eliminate 55 kDa heavy and ~23 kDa light chains from masking your proteins. Immunoprecipitation bead sets are also available for goat, mouse, and rabbit.TrueBlot® technology enables unhindered detection of protein bands of interest within IP western blot protocols which would otherwise be obscured by the presence of reduced & denatured heavy and light chain immunoglobulin in the IP blot (as detected by the conventional immunoblotting HRP anti-rabbit IgG reagent). TrueBlot® is the unique horseradish peroxidase conjugated anti-rabbit IgG immunoblotting second step reagent which enables detection of immunoblotted target protein bands, without hindrance by interfering immunoglobulin heavy and light chains from your IP antibody. Use it in place of your usual HRP anti-rabbit IgG immunoblotting second step reagent. It is easy to generate publication-quality IP/WB data with Rabbit IgG TrueBlot®.
The basic TrueBlot® concept map is depicted below and outlines the major mechanisms by which TrueBlot® can create exceptionally clear results.
Rockland Immunochemicals Inc.Gilbertsville, PA 19525E-mail: email@example.comPhone: 800.656.7625