GFP Antibody Alkaline Phosphatase Conjugated
GFP Antibody Alkaline Phosphatase Conjugated
GFP Antibody Alkaline Phosphatase Conjugated - Dot Blot
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Datasheet

GFP Antibody Alkaline Phosphatase Conjugated

Goat Polyclonal

2 References
600-105-215
1 mg
WB, ELISA, Dot Blot
GFP, eGFP, rGFP
Goat
GFP Antibody Rhodamine Conjugated Pre-Adsorbed GFP Antibody GFP Antibody Fluorescein Conjugated Pre-adsorbed GFP Antibody Peroxidase Conjugate GFP (GOAT) Antibody Biotin Conjugated GFP Antibody Texas Red™ Conjugated Pre-Adsorbed GFP Antibody Dylight™ 488 Conjugated Pre-Adsorbed GFP Antibody Dylight™ 549 Conjugated Pre-Adsorbed GFP Antibody Dylight™ 649 Conjugated Pre-Adsorbed GFP Antibody Dylight™ 680 Conjugated Pre-Adsorbed GFP Antibody Dylight™ 800 Conjugated Pre-Adsorbed GFP Antibody Dylight™ 405 Conjugated Pre-Adsorbed
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Background

Conjugated GFP antibody is ideal for western blotting, ELISA and Immunohistochemistry. Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.

Product Details

Anti-GFP (GOAT) Antibody Alkaline Phosphatase Conjugated - 600-105-215
goat anti-GFP Antibody alkaline phosphatase Conjugation, alk phos conjugated goat anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish
Goat
Alkaline Phosphatase (AP)
Polyclonal
IgG

Target Details

GFP, eGFP, rGFP
Recombinant Protein
Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Anti-GFP Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Alkaline Phosphatase and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum.  No reaction was observed against Human, Mouse and Rat Serum Proteins.
UniProtKB - P42212

Application Details

Dot Blot, ELISA, WB
Anti-GFP is designed to detect GFP and its variants. Anti-GFP Alkaline Phosphatase conjugated antibody has been tested by ELISA, dot blot, and western blot. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with Rockland's monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP (code # S000-03). Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots

Formulation

Liquid (sterile filtered)
1.0 mg/mL by UV absorbance at 280 nm
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
0.1% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Shipping & Handling

Wet Ice
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Expiration date is one (1) year from date of receipt.
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Applications
WB, IB, PCA; Receptor Binding Assays
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Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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