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Datasheet

Sars Nucleocapsid Protein Antibody

Rabbit Polyclonal

76 References
200-401-A50
500 µg
WB, ELISA, IHC, IF, FC, FISH, LFA, Multiplex, Other
Virus
Rabbit
$525.00 /Per Item
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Background

The coronavirus nucleocapsid protein is the major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid.  Sequence comparison of the N genes of five strains of the coronavirus mouse hepatitis virus suggests a three-domain structure for the nucleocapsid protein. Anti-SARS-CoV Nucleocapsid (N) Protein Antibody is useful for researchers interested in viral research.

Product Details

Anti-SARS-CoV Nucleocapsid (N) Protein (RABBIT) Antibody - 200-401-A50
rabbit anti-Sars Nucleocapsid Protein Antibody, rabbit anti-Sars-CoV Nucleocapsid (N) Protein Antibody, N antibody, N structural protein antibody, NC antibody, Nucleocapsid protein antibody, Nucleoprotein antibody, SARS coronavirus N protein antibody, SARS CoV antibody, SARSCoV antibody, Severe acute respiratory syndrome antibody, A50 Antibody, A50 SARS, COVID
Rabbit
Polyclonal
IgG

Target Details

Virus
Recombinant Protein
This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a purified recombinant protein corresponding to full length SARS Coronavirus Nucleocapsid protein. Lifesensors Inc. (www.lifesensors.com) prepared the Nucleocapsid protein as follows: SUMO-Nucleocapsid fusion was expressed in E.coli in LB medium and purified using Ni-NTA resin (Qiagen) affinity chromatography. After the fusion was cleaved by the SUMO Protease (LifeSensors), the SUMO tag and protease were subtracted from the nucleocapsid using MAC and the nucleocapsid was finally purified using Cation Exchange Chromatography with the Macro-Prep High S resin (BioRad) and size exclusion chromatography.
This protein A purified antibody is directed against SARS Coronavirus Nucleocapsid (N) protein. The product was purified from monospecific antiserum by protein A affinity purification.  Detects the Nucleocapsid (N), Omicron, and BA.2 sub-variant. BLAST analysis was used to suggest reactivity with related Coronavirus proteins.  Cross reactivity with homologues from other sources has not been determined.

Application Details

ELISA, WB
FC, FISH, IF, IHC, Other, LFA, Multiplex  - View References
This protein A purified antibody has been tested for use in ELISA, western blot, Immunohistochemistry, Immunofluorescence, and lateral flow.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 46 kDa in size corresponding to SARS Nucleocapsid (N) protein by western blotting in the appropriate cell lysate or extract. ELISA and lateral flow format has been used to detect virus in extracts from nasal and throat swabs and saliva. IF has been used to determine the presence or absence of virus entering cells especially when anti-viral drugs are applied. IHC studies have been performed on biopsies, included retrospective studies on cadaver tissues after formalin fixation and paraffin embedding, detecting the coronavirus in lung, liver, bile duct, and placenta tissue. Yet other studies have shown this antibody has the ability to neutralize the virus and thereby protect cells from the uptake of live virus. Others have demonstrated the utility of the antibody in flow cytometry studies.

Formulation

Lyophilized
5.3 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
100 µL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
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Lateral Flow (LFA)
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IF, Confocal Microscopy
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EIA, ELISA
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IHC, ICC, Histology
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ISH, FISH
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WB, IB, PCA
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IF, Confocal Microscopy
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IHC, ICC, Histology
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IHC, ICC, Histology
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Lateral Flow (LFA)
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IF, Confocal Microscopy; Multiplex Assay
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IF, Confocal Microscopy
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IF, Confocal Microscopy
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IF, Confocal Microscopy
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WB, IB, PCA
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IF, Confocal Microscopy; FC, FACS, FLOW
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IF, Confocal Microscopy
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IF, Confocal Microscopy
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IF, Confocal Microscopy
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IF, Confocal Microscopy
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Other
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IF, Confocal Microscopy
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IF, Confocal Microscopy
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Undefined
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IF, Confocal Microscopy
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Applications
IF, Confocal Microscopy

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