Datasheet

HL-60 Cell Nuclear Extract

Name: HL-60 Cell Nuclear Extract
Brand: Rockland Immunochemicals
Price: 210.0 USD
Availability: OutOfStock

Item No. W09-001-GK9

Size: 200 µg

Origin: Human

$210.00 /Per Item

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Background

Multi-purpose HL-60 nuclear extracts produced by Rockland Immunochemicals are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels.

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Specifications for HL-60 Cell Nuclear Extract

Product Details

Description: HL-60 Cell Nuclear Extract - W09-001-GK9

Synonyms: HL-60 lysate nuclear extract, HL60 nuclear lysate, HL-60 Nuclear lysate

Species of Origin: Human

Target Details

Purity/Specificity: HL-60 cells were grown in Ioscove's medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer to lyse the cells. Protein integrity was ensured using a cocktail of protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases (0.1 mM AEBSF HCl, 0.08 µM Aprotinin, 5 µM Bestatin, 1.5 µM E-64, 2 µM Leupeptin Hemisulfate, 1 µM Pepstatin A). Phosphatase inhibitors 1 mM NaF and 1 mM Na3VO4 were also added. Cell debris was removed by centrifugation. Protein concentration was determined by a modified Lowry assay using a commercially available kit. Protein concentration was adjusted to 4 mg/ml in RIPA buffer containing protease and phosphatase inhibitors.

Application Details

Application Note: Multi-purpose HL-60 nuclear extracts are especially prepared as positive control for multiple assays including western blot, immunoprecipitation (IP), capture ELISA or other assays requiring native protein sample. For separation by SDS-PAGE and subsequent western blot analysis, lysates should be diluted by user to desired concentration in SDS-PAGE buffer with 2-mercaptoethanol or dithiothreitol as the reducing agent and heated to 95° C for 5 minutes. Sample is ready for use in immunoprecipitation and ELISA experiments, conditions should be optimized by the user. Rockland recommends its TrueBlot IP reagents for immunoprecipitation experiments.

Cell Line Data

Cell Line: Human - promyeloblast, acute promyelocytic leukemia

Lysate Fractionation: Nuclear Extract

Lysate Stimulation: Not Stimulated

Culture Type: Tissue Culture

Induction: None (Control)

Formulation

Physical State: Liquid (sterile filtered)

Concentration: 4.0 mg/mL by modified Lowry assay

Buffer: 1X RIPA Buffer with HALT Protease and Phosphatase Inhibitors

Preservative: None

Stabilizer: None

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -70° C or COLDER. For extended storage, aliquot Nuclear Extract to minimize freeze/thaw cycles.

Expiration: Expiration date is three (3) months from date of receipt.

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.