Datasheet

p62 Phospho T269/Ser272 Antibody

Name: p62 Phospho T269/Ser272 Antibody
Brand: Rockland Immunochemicals
Price: 475.0 USD
Availability: OutOfStock

Rabbit Polyclonal

Item No. 600-401-X19

Size: 100 µL

Applications: WB

Reactivity: Human

Host Species: Rabbit

$475.00 /Per Item

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Background

p62, also known as sequestosome1 (SQSTM1), is a shuttle protein transporting polyubiquitinated proteins for both proteasomal and lysosomal degradation. p62 is an integral component of inclusions in brains of various neurodegenerative disorders, including Alzheimer disease (AD) neurofibrillary tangles (NFTs) and Lewy bodies in Parkinson disease. p62 plays an important role in the protection of cells from the toxicity of misfolded proteins by enhancing aggregate formation especially in the later stages. Phosphorylation of Ser28 has recently been demonstrated to be related to the pathogenesis of Parkinson’s disease. p62 pT269/pS272 Antibody is ideal for researchers interested in neuroscience research.

Expand Background

Specifications for p62 Phospho T269/Ser272 Antibody

Product Details

Description: Anti-p62 pT269/Ser272 (RABBIT) Antibody - 600-401-X19

Synonyms: Sequestosome-1, EBI3-associated protein of 60 kDa, EBIAP, p60, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62

Host Species: Rabbit

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: SQSTM1 - View All SQSTM1 Products

Reactivity: Human

PTM Specificity: Phosphorylation

Immunogen Type: Conjugated Peptide

Immunogen: Anti-Phospho-Thr269/Ser272 p62 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Thr269/Ser272 of human p62 conjugated to KLH.

Purity/Specificity: Anti-Phospho-Thr269/Ser272 p62 Antibody is directed against p62 protein phosphorylated at T269 and S272. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Reactivity against p62 occurs from human sources. However, reactivity is also expected against non-human primate based on 100% sequence homology.

Database Links:

UniProtKB - Q13501

GeneID - 8878

NCBI - NP_003891.1

Application Details

Tested Applications: WB

Application Note: Anti-Phospho-Thr269/Ser272 p62 Antibody is tested for Western Blots and is specific for the ~62 kDa p62 protein phosphorylated at Thr269 and Ser272. Specific conditions for reactivity should be optimized by the end user.

Formulation

Physical State: Liquid (sterile filtered)

Concentration: Neat

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Preservative: None

Stabilizer: 0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Expiration: Expiration date is one (1) year from date of receipt.

Related Protocols

Adherent Cell Lysis Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.