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Primary Antibodies  >  Phycoerythrin Conjugated Antibodies
 

AKT1 PE Antibody

Mouse Monoclonal 5E5.F5.D7 IgG2a kappa
Code

200-308-J34
Size

50 µg
Price

$309.00
Availability

Ships next business day.
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Flow Cytometry of Mouse anti-AKT1 antibody.  
Cells:  LNCap Cells.
Stimulation:  none.
Primary antibody:  Phycoerythrin AKT1 antibody at 1.0 µg/mL for 20 min at 4°C.
Shipping & Handling Fees Apply.

Shipping:
$70.00 to United States
Handling:
$20.00
Synonyms: AKT 1 antibody, AKT-1, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 1 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma

AKT1 PE Antibody Properties

Description
Anti-AKT1 (MOUSE) Phycoerythrin Conjugated Monoclonal Antibody - 200-308-J34
Host
Mouse
Target Species
Human
Known Cross Reactivity
Human, Mouse, Rat
Clonality
Monoclonal 5E5.F5.D7 IgG2a kappa
Application
ELISA : User Optimized
IF Microscopy : User optimized
FlowCytometry : 1.0 µg/mL
Western Blot : User Optimized
Immunochemistry: User optimized
Physical State
Lyophilized
Shipping condition
Wet Ice
Format
IgG2a
FP Value
1-2 moles R-Phycoerythrin (RPE) per mole of IgG2a
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL to 20 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
None
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
GeneName
AKT1
Download CofA
Lot#  

AKT1 PE Antibody Description

Background
AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT1 (MOUSE) PE conjugated Monoclonal Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.
Immunogen
Anti-AKT1 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1 protein followed by monoclonal development.
Immunogen Type
Peptide
Storage Condition
Store vial at 4° C prior to restoration.   Restore with deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.   Expiration date is one (1) year from date of restoration.
Application Note
Anti-AKT1 PE Antibody is suitable for Flow Cytometry, ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT1 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.
Purity/Specifity
Anti-AKT1 antibody is directed against human AKT1. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT1 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT1 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Madhunapantula SV, et al.The Akt signaling pathway: An emerging therapeutic target in malignant melanoma. Cancer Biol Ther. 2011 Dec 15;12(12)
Specific Reference
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Product Type
Proteins and Peptides;
Catalog Number
000-000-401

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, FC
Reacts With
human, mouse, rat, chimpanzee
Catalog Number
200-301-401

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, FC
Reacts With
Human
Catalog Number
200-501-E71

Product Label

 

Description
R-Phycoerythrin (RPE)
Color
Red
Conjugation Reference
Modified from the method of Carlsson J, Drevin H, and Axen R. Protein thiolation and reversible protein-protein conjugation. Biochem J 173, 723-737 (1978).
Molecular Weight
240000
Excitation Wavelength
488
Conjugation Chemistry
SATA/MBS