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Primary Antibodies  >  Epigenetic Research Antibodies
 

Histone H4 K12-Ac Antibody

Rabbit Polyclonal
Code

600-401-P06
Size

50 µg
Price

$345.00
Availability

Ships next business day.
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Immunofluorescence of Histone H4 K12-Ac (RABBIT) Antibody:   Histone H4 K12-Ac antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Western Blot of Histone H4 K12-Ac (RABBIT) Antibody.  
Western Blot analysis against untreated cell extracts. 
Lane 1:  HeLa cell lysates.
Load:  35 µg per lane.
Primary antibody:  Histone H4 K12-Ac antibody at 1.0 µg/mL for overnight at 4°C.
Secondary antibody:  IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size:  11 kDa for Histone H4 K12-Ac (RABBIT) Antibody.
Other band(s): none.
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Shipping:
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Handling:
$20.00
Synonyms: Histone H4 K12-Ac, K12 acetylated, Acetyl-Histone H4 Antibody K12, acetylated antibodies, Anti-Histone H4K12ac Antibody, epigenetic antibodies, acetylated epigenetic antibodies, genetic memory antibodies, histone H4, HIST1H4A, H4/A, H4F4

Histone H4 K12-Ac Antibody Properties

Description
Anti-Histone H4 [ac Lys12] (RABBIT) Antibody - 600-401-P06
Host
Rabbit
Target Species
Human
Known Cross Reactivity
Human
Clonality
Polyclonal
Application
IF Microscopy : 1:10
Western Blot : 1:500-1:1000
Immunochemistry: 1:100
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
0.69 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
HIST4H4
Download CofA
Lot#  

Histone H4 K12-Ac Antibody Description

Background
Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, acetylation of H4 Lys5 (H4 Lys5Ac) has been linked to transcriptional activation and DNA repair. Newly assembled histones are typically acetylated on H4 at lysine 5 and 12. The enzyme histone acetyltransferase 1 (HAT1) is the primary modulator, and these marks are necessary for complete chromatin assembly. Research suggests that [ac Lys12] is associated with memory repair and telomere replication. Anti-Histone H4 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.
Immunogen
Histone H4 [ac Lys12] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic aceylated peptide surrounding Lysine 12 of human Histone H4.
Immunogen Type
Peptide
Post Translational Modification
Acetylation
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-Histone H4 K12 ac antibody is useful for Western Blot, Dot Blot, and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~11 kDa corresponding to Histone H4 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.
Purity/Specifity
Anti-Histone H4 [ac Lys12] was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Alfalfa based on 100% sequence homology. Cross-reactivity with Histone H4 [ac Lys12] from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Zhou BO, Wang S-S, Zhang Y, Fu X-H, Dang W, et al. (2011) Histone H4 Lysine 12 Acetylation Regulates Telomeric Heterochromatin Plasticity in Saccharomyces cerevisiae. PLoS Genet 7(1): e1001272. doi:10.1371/journal.pgen.1001272 Epigenetics and Cognitive Aging Altered Histone Acetylation Is Associated with Age-Dependent Memory Impairment in Mice Peleg, Shahaf; Sananbenesi, Farahnaz; Zovoilis, Athanasios; Burkhardt, Susanne; Bahari-Javan, Sanaz; Agis-Balboa, Roberto Carlos; Cota, Perla; Wittnam, Jessica Lee; Gogol-Doering, Andreas; Opitz, Lennart; Salinas-Riester, Gabriella; Dettenhofer, Markus; Kang, Hui; Farinelli, Laurent; Chen, Wei; Fischer, André. Science Magazine, 7 May 2010, Vol. 328 no. 5979 pp. 753-756.
Specific Reference
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Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-30

Product Type
Blood Products;
Catalog Number
D109-00-0100

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry