SDS PAGE Results of OspC Control Protein

Datasheet

OspC Control Protein

Name: OspC Control Protein
Brand: Rockland Immunochemicals
Price: 399.0 USD
Availability: InStock

2 References

Item No. 000-001-C11

Size: 100 µg

Applications: WB, SDS-PAGE

Origin: Borrelia burgdorferi

Expressed in: E. coli

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Background

Outer Surface Protein C, or OspC, is a 20.7 kDa immunogenic protein on the outer surface of the spirochete Borrelia burgdorferi. Its function is not known, but it is located with lipid-anchoring sites on the outer cell membrane. Lyme disease proteins are ideal for researchers interested in immunology, neurology, rheumatology, coinfections, autoimmune, and neurodegenerative diseases.

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Specifications for OspC Control Protein

Product Details

Description: OspC Control Protein - 000-001-C11

Synonyms: OspC, Borrelia burgdorferi OspC, PC, Outer Surface Protein C, control protein

Species of Origin: Borrelia burgdorferi

Expressed in: E. coli

Type: Recombinant Protein

Target Details

Gene Name: ospC, BB_B19 - View All ospC, BB_B19 Products

Purity/Specificity: OspC is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified OspC and was estimated to be greater than 90% pure.

Database Links:

UniProtKB - Q07337

NCBI - NP_047005.1

GeneID - 1194415

Application Details

Tested Applications: SDS-PAGE, WB

Application Note: OspC is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 63.1 kDa for OspC-MBP, (20.7 kDa for OspC and 42.4 for MBP) in size corresponding to OspC by Western blotting in the appropriate cell lysate or extract. Outer Surface Protein C has been tested in SDS-page and western blot.

Formulation

Physical State: Liquid (sterile filtered)

Concentration: 1.0 mg/mL by modified Lowry assay

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Expiration: Expiration date is six (6) months from date of receipt.

References (2)

Bowman KA. et al. (2024). Borrelia-specific antibody profiles and complement deposition in joint fluid distinguish antibiotic-refractory from -responsive Lyme arthritis. iScience.

Applications

Protein Assay

PubMed

Haslund-Gourley BS. et al. (2024). Host glycosylation of immunoglobulins impairs the immune response to acute Lyme disease. eBioMedicine.

Applications

Multiplex Assay

PubMed

Related Protocols

Adherent Cell Lysis Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.