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Proteins and Peptides  >  Purified Proteins
 

RAT IgG1 Kappa (κ) isotype control

Monoclonal RG1K IgG1 kappa
Code

012-001-330
Size

500 µg
Price

$165.00
Availability

Ships within 2 weeks.
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Shipping:
$70.00 to United States
Handling:
$20.00
Synonyms: Rat Isotype Control, Rat IgG1 isotype, Rat IgG1 kappa isotype

RAT IgG1 Kappa (κ) isotype control Properties

Description
RAT IgG1 Kappa (κ) isotype control - 012-001-330
Clonality
Monoclonal RG1K IgG1 kappa
Application
ELISA : User Optimized
FLISA : User Optimized
IF Microscopy : User Optimized
FlowCytometry : 1:1000-1:5000
Western Blot : User Optimized
Other Dilution: User Optimized
Species of Origin
Rat
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
Format
IgG1
PSC Type
Protein
PSC Format
Native Protein
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
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Lot#  

RAT IgG1 Kappa (κ) isotype control Description

Background
RAT IgG1 Kappa (?) isotype control is used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In Rat there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain a - IgA, ? - IgG 1, 2a, 2b, 2c and µ - IgM, light chain ? and ?.
Storage Condition
Store vial at 4° C prior to opening.   This product is stable 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.
Application Note
RAT IgG1 Kappa (?) isotype control can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.
Purity/Specifity
RAT IgG1 Kappa (?) isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G.  In an Ouchterlony double diffusion assay the material is non-reactive with antisera to rat IgG2a, IgG2b, IgG3 , IgM , and IgA.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat serum.  Light and heavy chain composition has been confirmed.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Proteins and Peptides;
Applications
ELISA, FLISA, WB, IF, FC
Catalog Number
010-001-330

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Type
Proteins and Peptides;
Applications
ELISA, FLISA, WB, IF, FC
Catalog Number
012-001-040

Product Type
Proteins and Peptides;
Applications
ELISA, FLISA, WB, IF, FC
Catalog Number
012-001-331

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry