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Primary Antibodies  >  Cancer Research Antibodies
 

Ly-6G/Ly-6C (Gr-1) Fluorescein Antibody

Rat Monoclonal RB6-8C5 IgG2b kappa
Code

200-502-L54
Size

500 µg
Price

$395.00
Availability

Ships within 2 weeks.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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Flow Cytometry of anti-Ly-6G/Ly-6C (Gr-1) Fluorescein Conjugated Monoclonal Antibody.
Cells: C57BL/6 mouse bone marrow.  
Stimulation:  none. 
Antibody:  CD11b PE and Fluorescein Anti-Ly-6G/Ly-6C (Gr-1) antibody (clone RB6-8C5).
Shipping & Handling Fees Apply.

Shipping:
$70.00 to United States
Handling:
$20.00
Synonyms: Ly-6G/Ly-6C (Gr-1), RB6-8C5, Gr-1, Ly-6G, Ly-6C

Ly-6G/Ly-6C (Gr-1) Fluorescein Antibody Properties

Description
Anti-Ly-6G/Ly-6C (Gr-1) (RAT) Fluorescein Conjugated Monoclonal Antibody - 200-502-L54
Host
Rat
Target Species
Mouse
Known Cross Reactivity
Mouse
Clonality
Monoclonal RB6-8C5 IgG2b kappa
Application
FlowCytometry : 10 µL/10^6 cells (0.1 µg)
Western Blot : User Optimized
Immunochemistry: User Optimized
ImmunoPrecipitation: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
Format
IgG2b
FP Value
4-6 moles Fluorescein (FITC) per mole of IgG2b
Concentration
0.5mg/mL Sufficient to run approximately 100 tests
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.09% (w/v) Sodium Azide
Stabilizer
None
GeneName
Gr-1
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Lot#  

Ly-6G/Ly-6C (Gr-1) Fluorescein Antibody Description

Background
Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. In bone marrow, the expression levels of Gr-1 directly correlate with granulocyte differentiation and maturation; Gr-1 is also transiently expressed on bone marrow cells in the monocyte lineage. Immature Myeloid Gr-1+ cells play a role in the development of antitumor immunity.
Immunogen
Anti-Ly-6G/Ly-6C (Gr-1) Antibody (Monoclonal) was produced by repeated immunizations with raised against granulocytes of mouse origin.
Immunogen Type
Other
Storage Condition
Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.
Application Note
Anti-Ly-6G/Ly-6C (Gr-1) is useful for Immunohistochemistry, Immunoprecipitation, Western Blot, and Flow Cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.
Purity/Specifity
Fluorescein conjugated Ly-6G/Ly-6C (Gr-1) Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse Ly-6G/Ly-6C (Gr-1). Cross reactivity with Ly-6G/Ly-6C (Gr-1) from other sources has not been tested. Anti-Ly-6G/Ly-6C (Gr-1) is conjugated with FITC under optimal conditions and the solution is free of unconjugated FITC.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Fleming TJ, et al. 1993. J. Immunol. 151:2399. Brummer E, et al. 1984. J. Leukocyte Biol. 36:505. Stoppacciaro A, et al. 1993. J. Exp. Med. 178:151. Tumpey TM, et al. 1996. J. Virol. 70:898. Czuprynski CJ, et al. 1994. J. Immunol. 152:1836. Nitta H, et al. 1997. Cell Vision 4:73. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. Engwerda CR, et al. 2004. Am. J. Pathol. 165:2123. Brown CR, et al. 2004. Infect. Immun. 72:4956. Andoniou CE, et al. 2005. Nature Immunology 6:1011. Li M, et al. 2006. P. Natl. Acad. Sci USA 103:11736. Dzhagalov I, et al. 2007. Blood 109:1620
Specific Reference
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Product Type
Proteins and Peptides;
Applications
ELISA, FLISA, WB, IF, FC
Catalog Number
010-001-334

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human, chimpanzee
Catalog Number
200-401-908

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-30

Product Label

 

Description
Fluorescein (FITC)
Color
Green
Conjugation Reference
Modified from The and Feltkamp, Immunology 18; 865, 1970.
Molecular Weight
390
Excitation Wavelength
495
Conjugation Chemistry
Isothiocyanate