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CD62L Fluorescein Antibody

Rat Monoclonal MEL-14 IgG2a kappa
NCI Collaboration
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Flow Cytometry of anti-CD62L Fluorescein Conjugated Monoclonal Antibody.
Cells:  C57Bl/6 splenocytes.  
Stimulation:  none. 
Antibody:  (Dotted Line) FITC Rat IgG2a isotype control; (Solid Blue Line) Fluorescein Anti-CD62L antibody using 0.25 ug.
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Number:
200-502-N87
Size:
500 µg
Price:
$285.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships within 2 weeks.

CD62L Fluorescein Antibody Properties

Description
Anti-CD62L (RAT) Fluorescein Conjugated Monoclonal Antibody - 200-502-N87
Host
Rat
Target Species
Mouse
Known Cross Reactivity
Mouse
Clonality
Monoclonal MEL-14 IgG2a kappa
Application
Flow Cytometry : 10 µL/10^6 cells (0.1 µg)
Immunohistochemistry: User Optimized
ImmunoPrecipitation: User Optimized
Other Dilution: Sufficient to run approximately 500 tests
Physical State
Liquid (sterile filtered)
Shipping Condition
Wet Ice
Format
IgG2a
FP Value
2-8 moles Fluorescein (FITC) per mole of IgG2a
Concentration
0.5mg/mL by UV absorbance at 280 nm
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.09% (w/v) Sodium Azide
Stabilizer
0.1% Gelatin
GeneName
Sell
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CD62L Fluorescein Antibody Description

Background
Anti-CD62L antibody is specific for mouse CD62L, also known as L-Selectin, a cell adhesion molecule which facilitates lymphocyte rolling on activated vascular endothelium and homing to high endothelial venules (HEV) as immune cells transmigrate from blood into peripheral tissues. L-Selectin is a member of a family of Selectin molecules which act together with the integrin family of adhesion molecules to mediate leukocyte-endothelial interactions. L-Selectin is characteristically expressed by neutrophils, and is also found on B cells, monocytes, granulocytes, and at varying levels on naive, effector and memory T cells. It is rapidly shed upon cell activation, releasing into the circulation a soluble form whose biological role is of particular interest in cancer biology research.The MEL-14 antibody may be used as a phenotypic marker for CD62L expression on a variety of immune cell types. Please note that CD62L (L-Selectin) itself is also referred to as MEL-14 in the literature.
Synonyms
L-selectin CD62 antigen-like family member L Leukocyte adhesion molecule 1 LAM-1 Leukocyte-endothelial cell adhesion molecule 1 LECAM1 Lymph node homing receptor Lymphocyte antigen 22 Ly-22 Lymphocyte surface MEL-14 antigen CD62L Sell Lnhr, Ly-22, Ly22
Immunogen
Anti-CD62L Antibody (Monoclonal) was produced by repeated immunizations with CD62L antigen.
Storage Condition
Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.
Application Note
Anti-CD62L is useful for Immunohistochemistry, Immunoprecipitation, and Flow Cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.
Purity/Specifity
Fluorescein conjugated CD62L Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD62L. Cross reactivity with CD62L from other sources has not been tested. Anti-CD62L is conjugated with FITC under optimal conditions and the solution is free of unconjugated FITC.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Lee L-F, Logronio K, Tu GH, Zhai W, Ni I, Mei L, Dilley J, Yu J, et al. 2012. Proc. Natl. Acad. Sci. 10.1073. Harp JR, Gilchrist MA, and Onami TM. 2010. J. Immunol. 185:5751-5761. Furukawa Y, Umemoto E, Jang MH, Tohya K, Miyasaka M, and Hirata T. 2008. J. Biol. Chem. 283: 12112-12119. Li Y, Brazzell J, Herrera A, and Walcheck B. 2006. 108: 2275-2279. Ochando JC, Yopp AC, Yng Y, Garin A, Li Y, Boros P, Llodra J, Ding Y, Lira SA, Krieger NR, and Bromberg JS. 2005. J. Immunol. 174: 6993-7005. Zhao L-C, Shey M, Farnsworth M, and Dailey MO. 2001. J. Biol. Chem. 276: 30631-30640. Suzuki A, Andrew DP, Gonzalo JA, Fukumoto M, Spellberg J, Hashiyama M, Takimoto H, Gerwin N, Webb I, Molineux G, Amakawa R, Tada Y, Wakeham A, Brown J, McNiece I, Ley K, Butcher EC, Suda T, Gutierrez-Ramos JC, and Mak TK. 1996. Blood. 87:3550-3562. Reichert RA, Jerabek L, Gallatin WM, Butcher EC, and Weissman IL. 1986. 136(10): 3535-3542.
Specific Reference
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Product Type
Proteins and Peptides;
Applications
ELISA, FLISA, WB, IF, FC
Catalog Number
010-001-332

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human, chimpanzee
Catalog Number
200-401-908

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-30

Product Label

 

Description
Fluorescein (FITC)
Color
Green
Conjugation Reference
Modified from The and Feltkamp, Immunology 18; 865, 1970.
Molecular Weight
390
Excitation Wavelength
495
Conjugation Chemistry
Isothiocyanate