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CD45RO Fluorescein Antibody

Mouse Monoclonal UCHL1 IgG2a kappa
NCI Collaboration
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Flow Cytometry of Mouse anti-CD45RO Fluorescein Conjugated Monoclonal Antibody.
Cells:  Human peripheral blood lymphocytes.  
Stimulation:  none. 
Antibody:  (Black dotted line) FITC Mouse IgG2a isotype control; (Blue) Fluorescein Anti-CD45RO mouse antibody using 5 ul (0.5ug).
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Number:
200-302-N71
Size:
500 µL
Price:
$185.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships within 2 weeks.

CD45RO Fluorescein Antibody Properties

Description
Anti-CD45RO (MOUSE) Fluorescein Conjugated Monoclonal Antibody - 200-302-N71
Host
Mouse
Target Species
Human
Known Cross Reactivity
Human
Clonality
Monoclonal UCHL1 IgG2a kappa
Application
IF Microscopy : User Optimized
Flow Cytometry : 5 uL/test/1x10e5 to 1x10e8 cells
Western Blot : User Optimized
Immunohistochemistry: User Optimized
ImmunoPrecipitation: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Wet Ice
Format
IgG2a
FP Value
2-8 moles Fluorescein (FITC) per mole of IgG2a
Concentration
0.1 mg/mL Sufficient to run approximately 100 tests
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.09% (w/v) Sodium Azide
Stabilizer
0.1% Gelatin
GeneName
PTPRC
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CD45RO Fluorescein Antibody Description

Background
The UCHL1 antibody reacts with the human CD45 isoform known as CD45RO, a protein tyrosine phosphatase of 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns. With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, and others.The UCHL1 antibody is widely used as a marker for human CD45RO expression on thymocytes, activated memory T cells, monocytes, macrophages and granulocytes. The antibody is reported to be cross-reactive for Chimpanzee CD45RO.
Synonyms
Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, PTPRC , CD45
Immunogen
Anti-CD45RO Antibody (Monoclonal) was produced by repeated immunizations with CD45RO antigen.
Storage Condition
Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.
Application Note
Anti-CD45RO is useful for Flow Cytometry, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, and Western Blot. Researchers should determine optimal titers for applications that are not stated.
Purity/Specifity
Fluorescein conjugated CD45RO Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD45RO. Reactivity is observed against human CD45RO. Cross reactivity is observed with Chimpanzee. Reactivity with CD45RO from other sources has not been tested. Anti-CD45RO is conjugated with FITC under optimal conditions and the solution is free of unconjugated FITC.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Imanguli MM, Swaim WD, League SC, Gress RE, Pavletic SZ, and Hakim FT. 2009. Blood. 113: 3620-3630. Di Carlo E, D’Antuono T, Pompa P, Giuliani R, Rosini S, Stuppia L, Musiani P, and Sorrentino C. 2009. Clin. Cancer Res. 15: 2979-2987. Kap YS, van Meurs M, van Driel N, Koopman G, Melief M-J, Brok HPM, Laman JD, and ‘t Hart BA. 2009. J.Histochemistry & Cytochemistry. 57: 1159-1167. Bonzheim I, Geissinger E, Tinguely M, Roth S, Grieb T, Reimer P, Wilhelm M, Rosenwald A, Muller-Hermelink HK, and Rudiger T. 2008. Am. J. Clin. Pathol. 130: 613-619. Kim M-H, Suh H-S, Si Q, Terman BE, and Lee SC. 2006. J. Virol. 80: 62-72. Cappione AJ, Pugh-Bernard AE, Anolik JH, and Sanz I. 2004. J. Immunol. 172: 4298-4307. Gougeon ML, Lecoeur H, Boudet F, Ledru E. Marzabal S, Boullier S, Roue R, Nagata S, and Heeney J. 1997. J. Immunol. 158: 2964-2976. Kulas DT, Freund GG, and Mooney RA. 1996. J. Biol. Chem. 271: 755-760.
Specific Reference
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Product Type
Proteins and Peptides;
Applications
ELISA, FLISA, WB, IF, FC
Catalog Number
010-001-332

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human, chimpanzee
Catalog Number
200-401-908

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-30

Product Label

 

Description
Fluorescein (FITC)
Color
Green
Conjugation Reference
Modified from The and Feltkamp, Immunology 18; 865, 1970.
Molecular Weight
390
Excitation Wavelength
495
Conjugation Chemistry
Isothiocyanate